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中的金属耐受性蛋白编码基因家族:全基因组鉴定、特征分析及多种金属胁迫下的表达

Metal Tolerance Protein Encoding Gene Family in : Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses.

作者信息

Li Zhiqiang, Wang Chenglong, Wang Kaiyi, Zhao Jiayu, Shao Jirong, Chen Hui, Zhou Meiliang, Zhu Xuemei

机构信息

College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China.

School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.

出版信息

Plants (Basel). 2022 Mar 23;11(7):850. doi: 10.3390/plants11070850.

Abstract

Metal tolerance proteins (MTP) as divalent cation transporters are essential for plant metal tolerance and homeostasis. However, the characterization and the definitive phylogeny of the MTP gene family in , and their roles in response to metal stress are still unknown. In the present study, MTP genes in were identified, and their phylogenetic relationships, structural characteristics, physicochemical parameters, as well as expression profiles under five metal stresses including Fe, Mn, Cu, Zn, and Cd were also investigated. Phylogenetic relationship analysis showed that 12 MTP genes were classified into three major clusters and seven groups. All s had typical structural features of the MTP gene family and were predicted to be located in the cell vacuole. The upstream region of s contained abundant cis-acting elements, implying their functions in development progress and stress response. Tissue-specific expression analysis results indicated the regulation of s in the growth and development of . Besides, the expression of most genes could be induced by multiple metals and showed different expression patterns under at least two metal stresses. These findings provide useful information for the research of the metal tolerance mechanism and genetic improvement of .

摘要

金属耐受蛋白(MTP)作为二价阳离子转运蛋白,对于植物的金属耐受性和体内稳态至关重要。然而,[具体植物名称]中MTP基因家族的特征、确切系统发育以及它们在响应金属胁迫中的作用仍不清楚。在本研究中,鉴定了[具体植物名称]中的MTP基因,并研究了它们的系统发育关系、结构特征、理化参数以及在包括铁、锰、铜、锌和镉在内的五种金属胁迫下的表达谱。系统发育关系分析表明,12个[具体植物名称]MTP基因被分为三个主要簇和七个组。所有[具体植物名称]MTP基因都具有MTP基因家族的典型结构特征,并预计定位于细胞液泡中。[具体植物名称]MTP基因的上游区域含有丰富的顺式作用元件,这暗示了它们在发育进程和胁迫响应中的功能。组织特异性表达分析结果表明[具体植物名称]MTP基因在[具体植物名称]生长发育中的调控作用。此外,大多数[具体植物名称]MTP基因的表达可被多种金属诱导,并且在至少两种金属胁迫下表现出不同的表达模式。这些发现为[具体植物名称]金属耐受机制的研究和遗传改良提供了有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d091/9003181/00a86101994b/plants-11-00850-g001.jpg

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