Kim Min-Kyoung, Park Geonha, Ji Yura, Lee Yun-Gyo, Choi Minsik, Go Seung-Hyeon, Son Miwon, Jang Young-Pyo
Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea.
Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Korea.
Plants (Basel). 2022 Mar 29;11(7):925. doi: 10.3390/plants11070925.
The flower buds of have been reported as a potent resource associated with anti-angiogenic, anti-tumor, anti-rheumatoid arthritis activities, as well as immunoregulation. This paper aimed to establish an optimal extraction method for flavonoids, as active phytochemicals, and to conduct a comparative analysis by profiling the different blooming stages. Optimized shaking extraction conditions from the design of experiments (DoE), such as minutely mixture design, 2 full factorial design, and polynomial regression analysis, involved an agitation speed of 150 rpm and temperature of 65 °C for 12 h in 56% (/) acetone solvent. After, a comparative analysis was performed on three blooming stages, juvenile bud, mature purple bud, and complete flowering, by ultra-high-performance liquid chromatography-photodiode array-mass spectrometry (UHPLC-PDA-MS). Most flavonoids increased during bud growth and then decreased when the bud opened for blooming. In particular, apigenin 7--glucuronide, genkwanin 5--primeveroside, and genkwanin strikingly showcased this pattern. Furthermore, the raw spectrometric dataset was subjected to orthogonal projection to latent structures discriminant analysis (OPLS-DA) to find significant differences in the flavonoids from the juvenile bud, mature purple bud, and complete flowering. In conclusion, the present study facilitates an understanding of flavonoid change at different blooming stages and provides a momentous reference in the research of .
已报道[植物名称]的花芽是一种与抗血管生成、抗肿瘤、抗类风湿性关节炎活性以及免疫调节相关的有效资源。本文旨在建立一种针对作为活性植物化学物质的黄酮类化合物的最佳提取方法，并通过对不同开花阶段进行分析来进行比较分析。从实验设计（DoE）中优化的振荡提取条件，如微小混合设计、二水平全因子设计和多项式回归分析，包括在56%（/）丙酮溶剂中以150 rpm的搅拌速度和65°C的温度提取12小时。之后，通过超高效液相色谱-光电二极管阵列-质谱联用仪（UHPLC-PDA-MS）对三个开花阶段，即幼芽、成熟紫芽和完全开花阶段进行了比较分析。大多数黄酮类化合物在芽生长过程中增加，然后在芽开放开花时减少。特别是芹菜素7-O-葡萄糖醛酸苷、芫花素5-O-樱草糖苷和芫花素显著呈现出这种模式。此外，对原始光谱数据集进行了正交投影到潜在结构判别分析（OPLS-DA），以找出幼芽、成熟紫芽和完全开花阶段黄酮类化合物的显著差异。总之，本研究有助于了解不同开花阶段黄酮类化合物的变化，并为[植物名称]的研究提供重要参考。
