Zheng Ting, Yu Xiaoxing, Sun Yongzheng, Zhang Qing, Zhang Xianwen, Tang Mengzhen, Lin Chaoyang, Shen Zhicheng
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China.
Hangzhou Ruifeng Biosciences Co., Ltd., 1500 Wenyi Road, Building 1, Room 103, Hangzhou 310000, China.
Plants (Basel). 2022 Mar 31;11(7):949. doi: 10.3390/plants11070949.
Bermuda grass () is notoriously difficult to control with some commonly used herbicides. We cloned a cytochrome P450 gene from Bermuda grass, named , which was found to confer tolerance to multiple herbicides in transgenic . These herbicides include: (1) acetolactate synthase (ALS) inhibitor herbicides nicosulfuron and penoxsulam; (2) p-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide mesotrione; (3) synthetic auxin herbicide dicamba; (4) photosynthesis inhibitor bentazon. We further generated transgenic soybean plants expressing , and found that these transgenic soybean plants gained robust tolerance to nicosulfuron, flazasulfuron, and 2,4-dichlorophenoxyacetic acid (2,4-D) in greenhouse assays. A field trial demonstrated that transgenic soybean is tolerant to flazasulfuron and 2,4-D at 4-fold and 2-fold the recommended rates, respectively. Furthermore, we also demonstrated that flazasulfuron and dicamba are much more rapidly degraded in vivo in the transgenic soybean than in non-transgenic soybean. Therefore, may be utilized for engineering transgenic crops for herbicide tolerance.
狗牙根（）很难用一些常用除草剂进行防治。我们从狗牙根中克隆了一个细胞色素P450基因，命名为，发现它能使转基因植物对多种除草剂产生耐受性。这些除草剂包括：（1）乙酰乳酸合成酶（ALS）抑制剂除草剂烟嘧磺隆和五氟磺草胺；（2）对羟基苯丙酮酸双加氧酶（HPPD）抑制性除草剂甲基磺草酮；（3）合成生长素除草剂麦草畏；（4）光合作用抑制剂灭草松。我们进一步培育了表达的转基因大豆植株，发现在温室试验中这些转基因大豆植株对烟嘧磺隆、氟嘧磺隆和2,4-二氯苯氧乙酸（2,4-D）具有很强的耐受性。一项田间试验表明，转基因大豆对氟嘧磺隆和2,4-D的耐受性分别为推荐用量的4倍和2倍。此外，我们还证明，与非转基因大豆相比，氟嘧磺隆和麦草畏在转基因大豆体内的降解速度要快得多。因此，可用于培育抗除草剂转基因作物。
