Australian Herbicide Resistance Initiative, School of Agriculture and Environment, University of Western Australia, Crawley, Australia.
Pest Manag Sci. 2020 May;76(5):1929-1937. doi: 10.1002/ps.5725. Epub 2020 Jan 3.
Relatively new herbicides that target 4-hydroxyphenylpyruvate dioxygenase (HPPD) are now available for use on the world's great grain crops (rice, wheat, corn and soybean) and for other uses. With widespread and persistent use of HPPD-inhibiting herbicides, the evolution of HPPD-inhibiting herbicide resistant weeds is inevitable. Currently, resistance to HPPD-inhibiting herbicides is known in two weed species, waterhemp and Palmer amaranth. Here, we report a HPPD-inhibiting herbicide resistant wild radish population from the Western Australia grain belt. This population was not selected with HPPD-inhibiting herbicides, rather it evolved resistance to earlier used herbicides with different modes of action and exhibits cross-resistance to HPPD-inhibiting herbicides.
Dose-response experiments showed the resistant (R) population exhibits 4 to 6.5-fold resistance to the HPPD-inhibiting herbicides mesotrione, tembotrione and isoxaflutole, compared to the susceptible (S) population. This resistance is not target-site based as cloning of full coding sequences of the HPPD genes from S and R plants did not reveal resistance-endowing single nucleotide polymorphisms. The HPPD gene expression levels are similar in S and R plants. In addition, no differences in [ C]-mesotrione uptake and translocation were observed in the S and R plants. However, the time required for R plants to metabolise 50% [ C]-mesotrione is 7.7-fold faster than for the S plants.
We confirm resistance to HPPD-inhibiting herbicides exists in a population of the economically damaging global weed wild radish. The resistance in this population is due to a non-target-site based enhanced rate of herbicide metabolism. © 2019 Society of Chemical Industry.
目前，世界上的主要粮食作物（水稻、小麦、玉米和大豆）和其他用途都可使用新型靶向 4- 羟苯基丙酮酸双加氧酶（HPPD）的除草剂。由于 HPPD 抑制剂类除草剂的广泛和持续使用，HPPD 抑制剂类除草剂抗性杂草的出现是不可避免的。目前，已有两种杂草物种（水花生和刺苋）对 HPPD 抑制剂类除草剂产生了抗性。在这里，我们报道了来自澳大利亚西部谷物带的一种对 HPPD 抑制剂类除草剂具有抗性的野生萝卜种群。该种群并未经过 HPPD 抑制剂类除草剂的选择，而是对具有不同作用模式的早期使用的除草剂产生了抗性，并对 HPPD 抑制剂类除草剂表现出交叉抗性。
剂量反应实验表明，与敏感（S）种群相比，抗性（R）种群对 HPPD 抑制剂类除草剂 mesotrione、tembotrione 和异恶唑草酮的抗性分别为 4 至 6.5 倍。这种抗性不是基于靶标位点的，因为从 S 和 R 植物克隆的完整 HPPD 基因编码序列未显示赋予抗性的单核苷酸多态性。S 和 R 植物的 HPPD 基因表达水平相似。此外，在 S 和 R 植物中，未观察到 [C]-mesotrione 摄取和转运的差异。然而，R 植物代谢 50%[C]-mesotrione 所需的时间比 S 植物快 7.7 倍。
我们证实了一种经济上有害的全球性杂草野生萝卜种群对 HPPD 抑制剂类除草剂产生了抗性。该种群的抗性是由于非靶标位点的基础上增强了除草剂代谢速率所致。 © 2019 化学工业协会。
