Wang Titi, Chen Wenmei, Shao Yanhong, Liu Jun, Tu Zongcai
College of Life Science, National R & D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Foods. 2022 Mar 29;11(7):988. doi: 10.3390/foods11070988.
The present study aims to investigate the effects of ultrasound on the non-covalent interaction of β-lactoglobulin (β-LG) and luteolin (LUT) and to investigate the relationship between allergenicity and human intestinal microbiota. After treatment, the conformational structures of β-LG were changed, which reflected by the decrease in α-helix content, intrinsic fluorescence intensity and surface hydrophobicity, whereas the β-sheet content increased. Molecular docking studies revealed the non-covalent interaction of β-LG and LUT by hydrogen bond, van der Walls bond and hydrophobic bond. β-LG-LUT complex treated by ultrasound has a lower IgG/IgE binding ability and inhibits the allergic reaction of KU812 cells, depending on the changes in the conformational epitopes of β-LG. Meanwhile, the β-LG-LUT complex affected the composition of human intestinal microbiota, such as the relative abundance of and . Therefore, ultrasound improved the non-covalent interaction of β-LG with LUT, and the reduction in allergenicity of β-LG depends on conformational epitopes and human intestinal microbiota changes.
本研究旨在探讨超声对β-乳球蛋白（β-LG）与木犀草素（LUT）非共价相互作用的影响，并研究致敏性与人类肠道微生物群之间的关系。处理后，β-LG的构象结构发生改变，表现为α-螺旋含量、内在荧光强度和表面疏水性降低，而β-折叠含量增加。分子对接研究揭示了β-LG与LUT通过氢键、范德华键和疏水键的非共价相互作用。超声处理的β-LG-LUT复合物具有较低的IgG/IgE结合能力，并抑制KU812细胞的过敏反应，这取决于β-LG构象表位的变化。同时，β-LG-LUT复合物影响人类肠道微生物群的组成，如 和 的相对丰度。因此，超声改善了β-LG与LUT的非共价相互作用，β-LG致敏性的降低取决于构象表位和人类肠道微生物群的变化。
