Saito Alexandre Yukio, Sussmann Rodrigo Antonio Ceschini, Kimura Emilia Akemi, Cassera Maria Belen, Katzin Alejandro Miguel
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
Department of Biochemistry and Virginia Tech Center for Drug Discovery, M/C 0308, Virginia Tech, Blacksburg, VA 24061, United States.
J Pharm Biomed Anal. 2015;111:100-3. doi: 10.1016/j.jpba.2015.03.030. Epub 2015 Apr 3.
Nerolidol is a naturally occurring sesquiterpene found in the essential oils of many types of flowers and plants. It is frequently used in cosmetics, as a food flavoring agent, and in cleaning products. In addition, nerolidol is used as a skin penetration enhancer for transdermal delivery of therapeutic drugs. However, nerolidol is hemolytic at low concentrations. A simple and fast GC-MS method was developed for preliminary quantification and assessment of biological interferences of nerolidol in mouse plasma after oral dosing. Calibration curves were linear in the concentration range of 0.010-5 μg/mL nerolidol in mouse plasma with correlation coefficients (r) greater than 0.99. Limits of detection and quantification were 0.0017 and 0.0035 μg/mL, respectively. The optimized method was successfully applied to the quantification of nerolidol in mouse plasma.
橙花叔醇是一种天然存在的倍半萜烯，存在于多种花卉和植物的精油中。它常用于化妆品、食品调味剂和清洁产品中。此外，橙花叔醇还用作治疗药物经皮递送的皮肤渗透促进剂。然而，橙花叔醇在低浓度下具有溶血作用。建立了一种简单快速的气相色谱-质谱联用（GC-MS）方法，用于口服给药后小鼠血浆中橙花叔醇的初步定量和生物干扰评估。在小鼠血浆中橙花叔醇浓度为0.010 - 5μg/mL范围内，校准曲线呈线性，相关系数（r）大于0.99。检测限和定量限分别为0.0017和0.0035μg/mL。该优化方法成功应用于小鼠血浆中橙花叔醇的定量分析。
