重组酶聚合酶扩增结合实时荧光探针检测
Recombinase Polymerase Amplification Combined with Real-Time Fluorescent Probe for Detection.
作者信息
Jiang Tingting, Wang Yacui, Jiao Weiwei, Song Yiqin, Zhao Qing, Wang Tianyi, Bi Jing, Shen Adong
机构信息
Baoding Key Laboratory for Precision Diagnosis and Treatment of Infectious Diseases in Children, Baoding Children's Hospital, Baoding 071051, China.
Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Disease, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
出版信息
J Clin Med. 2022 Mar 23;11(7):1780. doi: 10.3390/jcm11071780.
() is one of the major causes of community-acquired pneumonia, accounting for 20-40% of total cases. Rapid and accurate detection of is crucial for the diagnosis and rational selection of antibiotics. In this study, we set up a real-time recombinase polymerase amplification (RPA) assay to detect the conserved gene CARDS of . The amplification can be finished in 20 min at a wide temperature range from 37-41 °C. The limit of detection of RPA assay was 10 fg per microliter. Cross-reaction with commonly detected respiratory pathogens was not observed using RPA assay. Among clinical sputum samples, the detection rate of RPA assay and real-time PCR assay was 48.4% (92/190) and 46.3% (88/190), respectively ( = 0.68). Therefore, the RPA assay for detection is rapid and easy to use and may serve as a promising test for early diagnosis of infection.
（）是社区获得性肺炎的主要病因之一，占总病例的20-40%。快速准确地检测（）对于诊断和合理选择抗生素至关重要。在本研究中，我们建立了一种实时重组酶聚合酶扩增（RPA）检测方法来检测（）的保守基因CARDS。扩增可在37-41°C的宽温度范围内20分钟内完成。RPA检测的检测限为每微升10 fg。使用RPA检测未观察到与常见检测呼吸道病原体的交叉反应。在临床痰标本中，RPA检测和实时PCR检测的检出率分别为48.4%（92/190）和46.3%（88/190）（=0.68）。因此，用于检测（）的RPA检测快速且易于使用，可能作为（）感染早期诊断的一种有前景的检测方法。
Zotero 插件