Department of Chromatography, Faculty of Chemistry, University of M. Curie-Skłodowska, M. Curie-Skłodowska Sq. 3, 20-031 Lublin, Poland.
Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Krakow, Poland.
Molecules. 2022 Mar 25;27(7):2116. doi: 10.3390/molecules27072116.
The effect-directed detection (EDD) of fruit and leaves extracts was performed to assess their pharmacological properties. The EDD comprised TLC-direct bioautography against , a DPPH assay, as well as α-glucosidase, lipase, tyrosinase, and acetylcholinesterase (AChE) inhibition assays. The leaf extracts showed stronger antioxidant activity than the fruit extract as well as inhibition of tyrosinase and lipase. The fruit extract was found to be extremely active against and to inhibit α-glucosidase and AChE slightly more than the leaf extracts. UHPLC-MS/MS analysis was carried out for the bioactive fractions and pointed to the possible anti-dementia properties of the dibenzocyclooctadiene lignans found in the upper TLC fractions. Gomisin N (518 mg/100 g DW), schisanhenol (454 mg/100 g DW), gomisin G (197 mg/100 g DW), schisandrin A (167 mg/100 g DW), and gomisin O (150 mg/100 g DW) were the quantitatively dominant compounds in the fruit extract. In total, twenty-one lignans were found in the bioactive fractions.
采用效应导向检测（EDD）对果实和叶片提取物进行检测，以评估其药理特性。EDD 包括针对的 TLC 直接生物自显影、DPPH 测定以及α-葡萄糖苷酶、脂肪酶、酪氨酸酶和乙酰胆碱酯酶（AChE）抑制测定。叶片提取物的抗氧化活性强于果实提取物，并且对酪氨酸酶和脂肪酶具有抑制作用。与叶片提取物相比，发现果实提取物对具有极强的活性，并且对α-葡萄糖苷酶和 AChE 的抑制作用略强。对生物活性馏分进行了 UHPLC-MS/MS 分析，结果表明在上部 TLC 馏分中发现的二苯并环辛二烯木脂素具有抗痴呆特性。果实提取物中含量较高的化合物为：柯楠因 N（518mg/100gDW）、五味子酚（454mg/100gDW）、戈米辛 G（197mg/100gDW）、五味子甲素（167mg/100gDW）和戈米辛 O（150mg/100gDW）。在生物活性馏分中总共发现了二十一种木脂素。
