Microscopy Laboratory, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Crato 63105-000, CE, Brazil.
Laboratory of Microbiology and Molecular Biology, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Crato 63105-000, CE, Brazil.
Molecules. 2022 Mar 27;27(7):2169. doi: 10.3390/molecules27072169.
Weeds are an important source of natural products; with promising biological activity. This study investigated the anti-kinetoplastida potential (in vitro) to evaluate the cytotoxicity (in vitro) and antioxidant capacity of the essential oil of (EORe), which is an infesting plant species. The essential oil was analyzed by GC/MS. The antioxidant capacity was evaluated by reduction of the DPPH radical and Fe ion. The clone CL-B5 was used to search for anti-epimastigote activity. Antileishmanial activity was determined using promastigotes of (MHOM/CW/88/UA301). NCTC 929 fibroblasts were used for the cytotoxicity test. The results showed that the main constituent of the essential oil was γ-elemene. No relevant effect was observed concerning the ability to reduce the DPPH radical; only at the concentration of 480 μg/mL did the essential oil demonstrate a high reduction of Fe power. The oil was active against promastigotes; but not against the epimastigote form of . Cytotoxicity for mammalian cells was low at the active concentration capable of killing more than 70% of promastigote forms. The results revealed that the essential oil of showed activity against ; positioning itself as a promising agent for antileishmanial therapies.
杂草是天然产物的重要来源，具有有前景的生物活性。本研究调查了（EORe）的抗动基体（体外）潜力，以评估其具有细胞毒性（体外）和抗氧化能力，（EORe）是一种侵害性植物物种。通过 GC/MS 对精油进行分析。通过 DPPH 自由基和 Fe 离子的还原来评估抗氧化能力。使用 CL-B5 克隆来寻找抗前鞭毛体活性。使用 （MHOM/CW/88/UA301）的前鞭毛体来确定抗利什曼原虫活性。NCTC 929 成纤维细胞用于细胞毒性试验。结果表明，精油的主要成分是γ-榄香烯。在降低 DPPH 自由基的能力方面没有观察到相关作用；只有在 480μg/mL 的浓度下，精油才显示出对 Fe 离子的高还原能力。该油对 前鞭毛体具有活性；但对 的动基体形式无效。在能够杀死超过 70%的前鞭毛体形式的有效浓度下，对哺乳动物细胞的细胞毒性较低。结果表明， 的精油对 具有活性，为抗利什曼原虫疗法提供了有前景的药物。
