Faculty of Chemistry, Adam Mickiewicz University, 8: Uniwersytetu Poznańskiego, 61-614 Poznan, Poland.
Molecules. 2022 Mar 28;27(7):2202. doi: 10.3390/molecules27072202.
Solid lipid nanoparticles (SLNs) have been synthesized as potential drug delivery systems. They are classified as solid lipid nanocarriers that can successfully carry both hydrophilic and hydrophobic drugs. SLNs are based on a biocompatible lipid matrix that is enzymatically degraded into natural components found in the human body. Solid lipid nanoparticles are suitable for the incorporation of hydrophobic active ingredients such as curcumin. The study included the optimization of lipid nanoparticle composition, incorporation of the active compound (curcumin), a stability evaluation of the obtained nanocarriers and characterization of their lipid matrix. Through process optimization, a dispersion of solid lipid nanoparticles (solid lipid:surfactant—2:1.25 weight ratio) predisposed to the incorporation of curcumin was developed. The encapsulation efficiency of the active ingredient was determined to be 99.80%. In stability studies, it was found that the most suitable conditions for conducting high-pressure homogenization are 300 bar pressure, three cycles and a closed-loop system. This yields the required values of the physicochemical parameters (a particle size within a 200−450 nm range; a polydispersity index of <30%; and a zeta potential of about |±30 mV|). In this work, closed-loop high-pressure homogenization was used for the first time and compared to the currently preferred open-loop method.
固体脂质纳米粒（SLNs）已被合成作为潜在的药物传递系统。它们被归类为固体脂质纳米载体，可以成功携带亲水性和疏水性药物。SLNs 基于生物相容性脂质基质，该基质可被酶解为人体内存在的天然成分。固体脂质纳米粒适合包封疏水性活性成分，如姜黄素。本研究包括优化脂质纳米粒的组成、包封活性化合物（姜黄素）、对获得的纳米载体进行稳定性评估以及对其脂质基质进行表征。通过工艺优化，开发了一种有利于包封姜黄素的固体脂质纳米粒分散体（固体脂质：表面活性剂-2:1.25 重量比）。活性成分的包封效率确定为 99.80%。在稳定性研究中，发现进行高压匀浆的最适条件为 300 巴压力、三个循环和闭环系统。这产生了所需的物理化学参数值（粒径在 200-450nm 范围内；多分散指数<30%；和约 |±30 mV| 的 ζ 电位）。在这项工作中，首次使用闭环高压匀浆并与目前优选的开环方法进行了比较。
