Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, Tunisia.
Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France.
Molecules. 2022 Apr 2;27(7):2314. doi: 10.3390/molecules27072314.
Considering medicinal plants as an inexhaustible source of active ingredients that may be easily isolated using simple and inexpensive techniques, phytotherapy is becoming increasingly popular. Various experimental approaches and analytical methods have been used to demonstrate that the genus Calendula (Asteraceae) has a particular richness in active ingredients, especially phenolic compounds, which justifies the growing interest in scientific studies on this genus' species. From a chemical and biological viewpoint, is a little-studied plant. For the first time, high-performance liquid chromatography combined with negative electrospray ionization mass spectrometry (HPLC-HESI-MS) was used to analyze methanolic extracts of () fruits. Thirty-five molecules were identified. Flavonoids (47.87%), phenolic acids (5.18%), and saponins (6.47%) formed the majority of these chemicals. Rutin, caffeic acid hexoside, and Soyasaponin βg' were the most abundant molecules in the fruit methanolic extract, accounting for 17.49% of total flavonoids, 2.32 % of total phenolic acids, and 0.95% of total saponins, respectively. The antioxidant activity of the fruit extracts of was investigated using FRAP, TAC, and DPPH as well as flavonoids and total phenols content. Because the phenolic components were more extractable using polar solvents, the antioxidant activity of the methanolic extract was found to be higher than that of the dichloromethane and hexane extracts. The IC50 value for DPPH of methanolic extract was found to be 0.041 mg·mL. Our findings showed that is an important source of physiologically active compounds.
考虑到药用植物是一种活性成分的不竭来源，这些成分可以使用简单且廉价的技术轻易分离，因此植物疗法越来越受欢迎。各种实验方法和分析方法已被用于证明金盏花（菊科）属植物具有特别丰富的活性成分，特别是酚类化合物，这也解释了科学界对该属植物种的研究兴趣日益浓厚的原因。从化学和生物学的角度来看， 是一种研究较少的植物。首次使用高效液相色谱法结合负离子电喷雾电离质谱法（HPLC-HESI-MS）对 （）果实的甲醇提取物进行分析。鉴定出 35 种分子。这些化合物主要由类黄酮（47.87%）、酚酸（5.18%）和皂苷（6.47%）组成。芦丁、咖啡酸己糖苷和大豆皂苷 βg'是果实甲醇提取物中最丰富的分子，分别占总类黄酮的 17.49%、总酚酸的 2.32%和总皂苷的 0.95%。采用 FRAP、TAC 和 DPPH 以及类黄酮和总酚含量法研究了 果实提取物的抗氧化活性。由于极性溶剂更能提取酚类成分，因此发现甲醇提取物的抗氧化活性高于二氯甲烷和正己烷提取物。甲醇提取物对 DPPH 的 IC50 值为 0.041mg·mL。我们的研究结果表明， 是具有生理活性化合物的重要来源。
