Graduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba, João Pessoa 58051-900, Paraíba, Brazil.
Graduate Program in Ecology and Conservation, Department of Biology, Center for Biological and Health Sciences, Campina Grande 58429-500, Paraíba, Brazil.
Molecules. 2019 Jul 10;24(14):2519. doi: 10.3390/molecules24142519.
Vogel (Leguminosae) is a species popularly known in Brazil as "urinária", "urinana", and "carrapicho", it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in .
羽扇豆（豆科）在巴西俗称“urinária”、“urinana”和“carrapicho”，被广泛用作利尿剂和治疗性病的药物。本研究应用一种特定的方法，从羽扇豆中提取富含皂甙的部分，采用高效液相色谱法与二极管阵列检测、离子阱质谱和飞行时间质谱（HPLC-DAD-ESI-MS/MS）联用的方法分析三萜皂甙。MS 和 MS/MS 实验在负离子模式下进行。分子质量和碎片数据用于支持皂甙的结构表征。根据保留时间、高分辨率质量测定和碎片分析，在羽扇豆中初步鉴定出 35 种齐墩果烷三萜皂甙。
