Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
Cell Mol Biol (Noisy-le-grand). 2021 Jan 31;67(1):201-211. doi: 10.14715/cmb/2021.67.1.29.
Pyracantha coccinea M.Roem. is considered as an important medicinal plant contributing remarkably to health and medicinal benefits. This is attributed to the presence of abundant polyphenols with powerful antioxidant properties. However, little research has been studied on the comprehensive identification and characterization of the phenolic compounds in areal parts of P. coccinea. This study aimed to investigate, characterize, and quantify the phenolic profiles of P. coccinea through liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and high-performance liquid chromatography-photodiode array (HPLC-PDA. Further, it showed a significantly higher value in total phenolic content (TPC) than that of total flavonoids (TFC) and tannins (TTC). As for antioxidant capacities, P. coccinea presented the highest activity in ABTS (7.12 ± 0.25 mg AAE/g dw) compared with DPPH, FRAP, and TAC assays. The LC-ESI-QTOF-MS/MS analysis detected 28 phenolic compounds, including phenolic acids (12), flavonoids (13), other polyphenols (2), and lignans (1) in P. coccinea samples. The results from HPLC-PDA indicated the chlorogenic acid (11.49 ± 1.89 mg/g) was the most abundant phenolic acid, while kaempferol (14.67 ± 2.17 mg/g) was the predominant flavonoid in P. coccinea. This research confirms the benefits of the P. coccinea plant as a potential source of natural antioxidants for the food and pharmaceutical industries.
火棘被认为是一种重要的药用植物，对健康和药用功效有显著贡献。这归因于其丰富的多酚类物质，具有强大的抗氧化性能。然而，目前对于火棘地上部分的酚类化合物的综合鉴定和表征研究较少。本研究旨在通过液相色谱-电喷雾电离四极杆飞行时间质谱（LC-ESI-QTOF-MS/MS）和高效液相色谱-光电二极管阵列（HPLC-PDA）对火棘中的酚类物质进行鉴定、表征和定量分析。此外，它显示出总酚含量（TPC）显著高于总黄酮（TFC）和单宁（TTC）。在抗氧化能力方面，火棘在 ABTS（7.12 ± 0.25 mg AAE/g dw）中的活性最高，而 DPPH、FRAP 和 TAC 测定则较低。LC-ESI-QTOF-MS/MS 分析检测到 28 种酚类化合物，包括火棘样品中的酚酸（12 种）、黄酮类（13 种）、其他多酚类（2 种）和木脂素（1 种）。HPLC-PDA 的结果表明，绿原酸（11.49 ± 1.89 mg/g）是最丰富的酚酸，而山奈酚（14.67 ± 2.17 mg/g）是火棘中含量最高的黄酮类化合物。本研究证实了火棘植物作为食品和制药工业中天然抗氧化剂的潜在来源的益处。
