均匀分散在合理多孔碳纳米盒中的 CoO 纳米粒子，可显著增强对活细胞中释放的 HO 的电催化检测。

CoO Nanoparticles Uniformly Dispersed in Rational Porous Carbon Nano-Boxes for Significantly Enhanced Electrocatalytic Detection of HO Released from Living Cells.

机构信息

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing 400715, China.

Institute for Materials Science and Devices, School of Material Science and Engineering, Suzhou University of Science and Technology, Suzhou 215011, China.

出版信息

Int J Mol Sci. 2022 Mar 30;23(7):3799. doi: 10.3390/ijms23073799.

A facile and ingenious method to chemical etching-coordinating a metal-organic framework (MOF) followed by an annealing treatment was proposed to prepare CoO nanoparticles uniformly dispersed in rational porous carbon nano-boxes (CoO@CNBs), which was further used to detect HO released from living cells. The CoO@CNBs HO sensor delivers much higher sensitivity than non-etching/coordinating CoO, offering a limit of detection of 2.32 nM. The wide working range covers 10 nM-359 μM HO, while possessing good selectivity and excellent reproducibility. Moreover, this biosensor was used to successfully real-time detect HO released from living cells, including both healthy and tumor cells. The excellent performance holds great promise for CoO@CNBs's applications in electrochemical biomimetic sensing, particularly real-time monitor HO released from living cells.

提出了一种简便而巧妙的化学刻蚀-配位方法（金属-有机骨架（MOF），随后进行退火处理），以制备均匀分散在合理多孔碳纳米盒（CoO@CNBs）中的 CoO 纳米粒子，进一步用于检测活细胞中释放的 HO。CoO@CNBs HO 传感器的灵敏度比非刻蚀/配位 CoO 高得多，检测限为 2.32 nM。工作范围很宽，涵盖 10 nM-359 μM HO，同时具有良好的选择性和出色的重现性。此外，该生物传感器还成功地实时检测了活细胞中释放的 HO，包括健康细胞和肿瘤细胞。优异的性能为 CoO@CNBs 在电化学仿生传感中的应用提供了广阔的前景，特别是实时监测活细胞中释放的 HO。