Department of Nutrigenomics and Bromatology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland.
Department of Biochemistry, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
Int J Mol Sci. 2022 Mar 31;23(7):3859. doi: 10.3390/ijms23073859.
The induction of apoptosis is one of the main goals of the designed anti-cancer therapies. In recent years, increased attention has been paid to the physical factors such as magnetic fields and to the natural bioactive compounds and the possibilities using them in medicine. Hence, the aim of this study was to evaluate the anti-tumor effect of caffeic or chlorogenic acid in combination with a moderate-strength static magnetic field on C32 melanoma cells by assessing the effect of both factors on the apoptotic process. The apoptosis of the C32 cells was evaluated using a flow cytometry analysis. The expression of the apoptosis-associated genes was determined using the RT-qPCR technique. The caspase activity and the concentration of the oxidative damage markers were also measured. It was found that phenolic acids and a static magnetic field trigger the apoptosis of the C32 cells and also affect the expression of the genes encoding the apoptosis regulatory proteins. In conclusion, our study indicated that both of the phenolic acids and a static magnetic field can be used supportively in the treatment of melanoma and that caffeic acid is more pro-apoptotic than chlorogenic acid.
诱导细胞凋亡是设计抗癌疗法的主要目标之一。近年来，人们越来越关注磁场等物理因素以及天然生物活性化合物，并探讨将它们应用于医学的可能性。因此，本研究旨在通过评估两种因素对细胞凋亡过程的影响，评估咖啡酸或绿原酸与中强度静磁场联合应用对 C32 黑色素瘤细胞的抗肿瘤作用。采用流式细胞术分析评估 C32 细胞的凋亡情况。采用 RT-qPCR 技术测定与细胞凋亡相关基因的表达。还测量了半胱氨酸天冬氨酸蛋白酶的活性和氧化损伤标志物的浓度。结果表明，酚酸和静磁场可引发 C32 细胞凋亡，并影响编码凋亡调控蛋白的基因的表达。总之，我们的研究表明，两种酚酸和静磁场都可以在治疗黑色素瘤中得到支持，并且咖啡酸比绿原酸更具有促凋亡作用。
