Morgan D O, Roth R A
Proc Natl Acad Sci U S A. 1987 Jan;84(1):41-5. doi: 10.1073/pnas.84.1.41.
The role of the insulin receptor tyrosine kinase (protein-tyrosine kinase, EC 2.7.1.112) in various rapid insulin effects was studied by injecting four different cell types (by osmotic lysis of pinocytotic vesicles) with a monoclonal antibody that specifically inhibits the kinase activity of the insulin receptor and the closely related receptor for insulin-like growth factor (IGF)-I. Injection of this inhibitory antibody resulted in a decreased ability of insulin to stimulate the uptake of 2-deoxyglucose in Chinese hamster ovary cells and freshly isolated rat adipocytes, ribosomal protein S6 phosphorylation in CHO cells, and glycogen synthesis in the human hepatoma cell line HepG2. The ability of insulin, IGF-I, and IGF-II to stimulate glucose uptake in TA1 mouse adipocytes was also inhibited. Studies with CHO cells demonstrated that these effects of the inhibitory antibody were specific, since there was no change in phorbol ester-stimulated glucose uptake and injection of a noninhibiting antibody to the kinase had no effect on insulin action. These studies indicate that the tyrosine kinase activity of the insulin receptor is important in mediating several rapid insulin effects in a variety of different cell types.
通过向四种不同细胞类型（通过胞饮小泡的渗透裂解）注射一种特异性抑制胰岛素受体激酶活性以及与胰岛素样生长因子（IGF）-I密切相关受体激酶活性的单克隆抗体，研究了胰岛素受体酪氨酸激酶（蛋白酪氨酸激酶，EC 2.7.1.112）在各种快速胰岛素效应中的作用。注射这种抑制性抗体导致胰岛素刺激中国仓鼠卵巢细胞和新鲜分离的大鼠脂肪细胞摄取2-脱氧葡萄糖的能力降低，抑制CHO细胞中核糖体蛋白S6磷酸化以及人肝癌细胞系HepG2中的糖原合成。胰岛素、IGF-I和IGF-II刺激TA1小鼠脂肪细胞摄取葡萄糖的能力也受到抑制。对CHO细胞的研究表明，抑制性抗体的这些作用是特异性的，因为佛波酯刺激的葡萄糖摄取没有变化，并且注射针对该激酶的非抑制性抗体对胰岛素作用没有影响。这些研究表明，胰岛素受体的酪氨酸激酶活性在介导多种不同细胞类型中的几种快速胰岛素效应中很重要。
