Wright W E
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7822-6. doi: 10.1073/pnas.81.24.7822.
A selective system was developed that permits the isolation of heterokaryons and cell hybrids between any cell types without the use of selectable genetic markers. Antibodies were introduced into two populations of non-transformed normal diploid human fibroblasts by the osmotic lysis of pinosomes containing hypertonic antibody solutions. Heterokaryons formed by fusing antiricin-injected cells to antidiphtheria toxin-injected cells survived an overnight treatment in both ricin and diphtheria toxin. This approach should have general applicability to a variety of investigations such as the complementation analysis of human genetic syndromes, heterokaryon studies of cell differentiation, tumorigenesis and growth control, cell reconstruction experiments, and the production of hybridoma cells.
开发了一种选择性系统，该系统允许在不使用可选择遗传标记的情况下分离任何细胞类型之间的异核体和细胞杂种。通过含有高渗抗体溶液的胞饮小体的渗透裂解，将抗体引入两群未转化的正常二倍体人成纤维细胞中。将注射抗蓖麻毒素的细胞与注射抗白喉毒素的细胞融合形成的异核体在蓖麻毒素和白喉毒素中过夜处理后存活下来。这种方法应该对多种研究具有普遍适用性，例如人类遗传综合征的互补分析、细胞分化、肿瘤发生和生长控制的异核体研究、细胞重建实验以及杂交瘤细胞的产生。
