B4GALT5 高表达与肝癌预后不良相关。
B4GALT5 high expression associated with poor prognosis of hepatocellular carcinoma.
机构信息
Department of Radiotherapy, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.
Graduate School, Dalian Medical University, Dalian, China.
出版信息
BMC Cancer. 2022 Apr 12;22(1):392. doi: 10.1186/s12885-022-09442-2.
BACKGROUND
B4GALT5 is postulated to be an important protein in sugar metabolism that catalyzes the synthesis of lactosylceramide (LacCer). However, its role in hepatocellular carcinoma (HCC) remains unknown.
METHOD
We characterized the expression of B4GALT5 in HCC tissue compared to normal tissue, and explored its function of B4GALT5 in HCC by enrichment analysis based on its co-expressed gene set. Next, we checked whether B4GALT5 expression is correlated to immune infiltration level and clinical prognosis in hepatocellular carcinoma. Finally, we verified the expression of B4GALT5 using clinical samples evaluated by RT-PCR, and conducted in vitro experiments with B4GALT5-knockdown HCC cells to investigate the function of B4GALT5 in the HCC cell proliferation, migration and invasion.
RESULTS
We found B4GALT5 mRNA and protein expression levels were significantly high in HCC tissue compared to normal tissue. The enrichment analysis of the gene sets that co-expressed with B4GALT5 showed specificity in HCC-related pathways and functions. Also, the expression pattern of B4GALT5 was significantly related to the immune infiltration level, especially CD4+ T cell and macrophage cells. B4GALT5 higher mRNA expression was associated with poor overall survival (OS) in HCC patients. Furthermore, In vitro experiments showed that depletion of B4GALT5 significantly inhibited HCC cell proliferation, migration and invasion. This study revealed the function and its mediated pathways of B4GALT5 in HCC, indicating that B4GALT5 may serve as a prognostic biomarker of HCC.
背景
B4GALT5 被推测是糖代谢中的一种重要蛋白质，它催化乳糖基神经酰胺（LacCer）的合成。然而，它在肝细胞癌（HCC）中的作用尚不清楚。
方法
我们比较了 HCC 组织和正常组织中 B4GALT5 的表达，并基于其共表达基因集进行富集分析，探讨了 B4GALT5 在 HCC 中的功能。接下来，我们检查了 B4GALT5 的表达是否与 HCC 中的免疫浸润水平和临床预后相关。最后，我们使用 RT-PCR 评估的临床样本验证了 B4GALT5 的表达，并通过 B4GALT5 敲低 HCC 细胞进行了体外实验，以研究 B4GALT5 在 HCC 细胞增殖、迁移和侵袭中的功能。
结果
我们发现与正常组织相比，B4GALT5 mRNA 和蛋白表达水平在 HCC 组织中明显升高。与 B4GALT5 共表达的基因集的富集分析显示出与 HCC 相关途径和功能的特异性。此外，B4GALT5 的表达模式与免疫浸润水平显著相关，尤其是 CD4+T 细胞和巨噬细胞。B4GALT5 的高 mRNA 表达与 HCC 患者的总生存期（OS）不良显著相关。此外，体外实验表明，B4GALT5 的耗竭显著抑制了 HCC 细胞的增殖、迁移和侵袭。这项研究揭示了 B4GALT5 在 HCC 中的功能及其介导的途径，表明 B4GALT5 可能作为 HCC 的预后生物标志物。
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