Taxonomy of Angiosperms and Biosystematics Laboratory, Department of Botany, University of North Bengal, Darjeeling, West Bengal, India.
Department of Botany, University of North Bengal, Darjeeling, West Bengal, India.
Microsc Res Tech. 2022 Jul;85(7):2628-2650. doi: 10.1002/jemt.24118. Epub 2022 Apr 12.
Pollen grains of 58 wetland species of angiosperms belonging to 17 families, growing in different wetlands of Sub-Himalayan biodiversity hotspots, Northern Bengal and lower Gangetic plains, India were investigated during a 4-year span of 2015-2019. Freshly collected anthers were processed through the acetolysis method and the obtained pollen grains were accurately studied under LM and SEM and properly photographed. Different attributes viz. shape, size, aperture type and exine ornamentation of pollen grains served as consistent features were used for authentic identification of the studied species. To study the phylogeny of pollen grains, characters like polarity, symmetry, aperture and exine sculpturing were found most significant for species segregation. Investigated pollen grains were mostly prolate-spheroidal, spheroidal or triangular, rarely prolate or elongated, exine psilate, granulate, reticulate or microechinate type. Most of the observable aperture of pollen grains of studied species were colporate, colpate and porate, while ulcerate and inaperturate grains were also observed in the studied aquatic members of the families Cyperaceae and Potamogetonaceae. All the gathered data were further statistically analyzed through ANOVA, PCA and Pearson Correlation Matrix to understand the interrelationship among the species. The permanent slides of identified pollen grains were deposited at the herbarium of North Bengal University [NBU] for future references. HIGHLIGHTS: Study of Pollen morphology of 58 wetland species under light & scanning electron microscopy. Unique apertures and exine ornamentation used to understand taxonomic evaluation of studied species. Pollen character supports interrelationship among the studied species. Relationship between exine thickness and the habitat of the wetland plants.
对生长在印度喜马拉雅生物多样性热点地区、北孟加拉和恒河下游平原不同湿地的 58 种被子植物的花粉粒进行了研究，这些植物属于 17 科。在 2015 年至 2019 年的 4 年期间，对新鲜采集的花药进行了乙酰解处理，然后通过 LM 和 SEM 对获得的花粉粒进行了精确研究，并进行了适当的拍摄。花粉粒的不同特征，如形状、大小、孔型和外壁纹饰，被用作鉴定研究物种的可靠特征。为了研究花粉粒的系统发育，极性、对称性、孔型和外壁纹饰等特征被发现对物种分离最为重要。研究的花粉粒大多为长球形、球形或三角形，很少为长形或伸长形，外壁纹饰为平滑、颗粒状、网状或微刺状。研究物种的花粉粒的可观察孔大多为沟孔、孔沟和多孔，而在研究的 Cyperaceae 和 Potamogetonaceae 科的水生成员中也观察到了溃疡孔和无孔。所有收集的数据都通过方差分析（ANOVA）、主成分分析（PCA）和 Pearson 相关矩阵进行了进一步的统计分析，以了解物种之间的相互关系。鉴定的花粉粒的永久载玻片被存放在北孟加拉大学（NBU）的植物标本馆中，以备将来参考。重点：通过光镜和扫描电子显微镜研究 58 种湿地物种的花粉形态。独特的孔型和外壁纹饰用于理解研究物种的分类评价。花粉特征支持研究物种之间的相互关系。外壁厚度与湿地植物栖息地之间的关系。
