Dasanayake Randima, Balendran Thivya, Atapattu Dhilma, Iddawela Devika
Animal Quarantine Office, Bandaranayake International Airport, Katunayake, Sri Lanka.
Department of Parasitology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
BMC Res Notes. 2022 Apr 14;15(1):137. doi: 10.1186/s13104-022-06024-0.
Canine dirofilariasis is a mosquito borne zoonotic filarial parasitic disease caused by Dirofilaria species. In Sri Lanka, human dirofilariasis is endemic and well documented. Even though canine dirofilariasis is an established infection among dog populations in Sri Lanka, the prevalence and risk factors were not documented in Kanthale divisional secretariat, Eastern Province of Sri Lanka. Therefore, the main objective of this study is to determine the prevalence of dirofilariasis and to identify the exposure-related risk factors in dogs using an interviewer administered questionnaire in Kanthale divisional secretariat.
Out of 162 blood samples collected from dogs, 47.5% (n = 77/162) were positive for the presence of microfilariae by direct smear. Of 162 samples 58.6% (n = 95/162) were molecularly identified as D. repens. Species-specific primer pair DIR3/DIR4 that amplified 5S rRNA was used. The current study observed a significant association between mongrels and canine dirofilariasis (p = 0.049), where 61.3% (n = 95) out of 155 mongrels showed PCR positivity. This study showed no significant association between the occurrence of dirofilariasis and the age, sex, rearing method, presence or absence of skin rash, and stray or domestic dogs. Dirofilaria immitis was not identified in direct smear test and PCR.
犬恶丝虫病是一种由恶丝虫属引起的、通过蚊子传播的人兽共患丝虫寄生虫病。在斯里兰卡，人类恶丝虫病呈地方性流行且有充分记录。尽管犬恶丝虫病在斯里兰卡犬类群体中是一种已确诊的感染，但在斯里兰卡东部省坎塔勒分区秘书处，其患病率和危险因素尚未有记录。因此，本研究的主要目的是通过在坎塔勒分区秘书处使用访谈式问卷来确定犬恶丝虫病的患病率，并识别犬类中与暴露相关的危险因素。
从犬类采集的162份血液样本中，直接涂片法检测微丝蚴阳性率为47.5%（n = 77/162）。在162份样本中，58.6%（n = 95/162）通过分子方法鉴定为匐行恶丝虫。使用扩增5S rRNA的物种特异性引物对DIR3/DIR4。本研究观察到杂种犬与犬恶丝虫病之间存在显著关联（p = 0.049），155只杂种犬中有61.3%（n = 95）PCR检测呈阳性。本研究表明，恶丝虫病的发生与年龄、性别、饲养方式、是否有皮疹以及流浪犬或家养犬之间无显著关联。在直接涂片试验和PCR中均未鉴定出犬恶丝虫。
