Clark D R, Hajar T M
Clin Chem. 1987 Jan;33(1):118-9.
Methylecgonine is a common metabolite of cocaine in man. We prepared methylecgonine and developed thin-layer chromatographic and gas-chromatographic methods for its detection in urine. Seventy urine specimens from our drug screening laboratory were tested by our method and by EMIT. Both methods were positive for 26 urines, and both were negative for 42 urines. The other two urines were shown to contain cocaine by GC/MS, and no detectable metabolites. We thus demonstrated that detection of methylecgonine and cocaine is as sensitive a test for cocaine use as EMIT.
甲基芽子碱是人体中可卡因的常见代谢产物。我们制备了甲基芽子碱，并开发了用于在尿液中检测它的薄层色谱法和气相色谱法。我们用自己的方法和酶放大免疫测定技术（EMIT）对来自我们药物筛查实验室的70份尿液标本进行了检测。两种方法对26份尿液检测结果呈阳性，对42份尿液检测结果呈阴性。另外两份尿液经气相色谱-质谱联用仪（GC/MS）检测显示含有可卡因，但无可检测到的代谢产物。因此，我们证明了甲基芽子碱和可卡因的检测对于可卡因使用的检测与EMIT一样灵敏。
