Valera Ane, Wang Shan, Carr Reuben, Trembleau Laurent, Deng Hai
Department of Chemistry, University of Aberdeen Aberdeen AB24 3UE Scotland UK
Ingenza Ltd Scotland UK.
RSC Adv. 2022 Apr 6;12(17):10540-10544. doi: 10.1039/d1ra08876a. eCollection 2022 Mar 31.
ketol-acid reductoisomerases have been widely studied due to their metabolic importance towards development of drug-resistant bacteria treatment. We here report the biochemical characterization of a new KARI (MtKARI-II) from a variant with a similar kinetic profile to class I KARIs. Phylogenetic analysis suggested that MtKARI-II is clustered into a class II KARI superfamily.
由于酮醇酸还原异构酶在耐药细菌治疗药物开发中的代谢重要性,它们已得到广泛研究。我们在此报告了一种来自变体的新型KARI(MtKARI-II)的生化特性,其具有与I类KARIs相似的动力学特征。系统发育分析表明,MtKARI-II聚类到II类KARI超家族中。