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具有类还原酶活性的纳米酶：抗氧化特性与电化学行为

Nanozymes with reductase-like activities: antioxidant properties and electrochemical behavior.

作者信息

Stasyuk Nataliya, Gayda Galina, Kavetskyy Taras, Gonchar Mykhailo

机构信息

Institute of Cell Biology, National Academy of Sciences of Ukraine 79005 Lviv Ukraine

Drohobych Ivan Franko State Pedagogical University 82100 Drohobych Ukraine

出版信息

RSC Adv. 2022 Jan 12;12(4):2026-2035. doi: 10.1039/d1ra08127f.

DOI:10.1039/d1ra08127f
PMID:35425252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8979041/
Abstract

Nanozymes (NZs) as stable cost-effective mimics of natural enzymes may be promising catalysts in food and environmental biotechnology, biosensors, alternative energy and medicine. The majority of known NZs are mimetics of oxidoreductases, although there are only limited data regarding mimetics of reductases. In the present research, a number of metal-based NZs were synthesized chemical methods and screened for their antioxidant ability in solution. The most effective reductase-like Zn/Cd/Cu NZ was characterized in detail. Its antioxidant properties in comparison with several food products and Trolox, as well as substrate specificity, size and composition were studied. Zn/Cd/Cu NZ was shown to mimic preferentially selenite reductase. The amperometric sensor was constructed possessing a high sensitivity (1700 A M m) and a broad linear range (16-1000 μM) for selenite ions. The possibility to apply the fabricated sensor for selenite determination in commercial mineral water has been demonstrated.

摘要

纳米酶（NZs）作为天然酶的稳定且经济高效的模拟物，在食品和环境生物技术、生物传感器、替代能源及医学领域可能是很有前景的催化剂。尽管关于还原酶模拟物的数据有限，但大多数已知的纳米酶是氧化还原酶的模拟物。在本研究中，通过化学方法合成了多种金属基纳米酶，并在溶液中筛选了它们的抗氧化能力。对最有效的类还原酶Zn/Cd/Cu纳米酶进行了详细表征。研究了其与几种食品和Trolox相比的抗氧化性能，以及底物特异性、尺寸和组成。结果表明，Zn/Cd/Cu纳米酶优先模拟亚硒酸盐还原酶。构建的安培传感器对亚硒酸根离子具有高灵敏度（1700 A M m）和宽线性范围（16 - 1000 μM）。已证明所制备的传感器可用于测定市售矿泉水中的亚硒酸盐。

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ACS Appl Bio Mater. 2021 Apr 19;4(4):3539-3546. doi: 10.1021/acsabm.1c00069. Epub 2021 Mar 25.
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Electrochemical Determination of Antioxidant Capacity of Traditional Homemade Fruit Vinegars Produced with Double Spontaneous Fermentation.双自发发酵法酿造传统自制果醋抗氧化能力的电化学测定
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RSC Adv. 2022 Aug 2;12(33):21309-21317. doi: 10.1039/d2ra02106d. eCollection 2022 Jul 21.
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