Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Environment and Biotechnology Research Division, Research Institute of Petroleum Industry, Tehran, Iran.
Sci Rep. 2022 Apr 15;12(1):6260. doi: 10.1038/s41598-022-10430-6.
Hydrogen sulfide (HS) is a super toxic substance that produces SO gases when combusted. Therefore, it should be removed from gas streams. Biodesulfurization is one of the developing methods for removing sulfide. Gas biodesulfurization must be accelerated to be competitive with chemical processes. This process has two sides: biotic and abiotic sides. To increase the rate of sulfide removal, this substance should be given to the bacteria in the maximum amount (Max. - R). Therefore, it is necessary to minimize the rate of adverse abiotic reactions of sulfide (Min. - R). Minimizing the sulfide reaction with biosulfur and oxygen and thiosulfate generation (Min. - R) was assessed in de-microbized medium. It was concluded that the pH should be kept as low as possible. The kinetics of thiosulfate formation from sulfide oxidation (- R) are strongly dependent on the sulfide concentration, and to minimize this reaction rate, sulfide should be gently injected into the culture. To minimize sulfide reduction to hydrogen sulfide (Min. - R), the pH should be kept as high as possible. Using the Design Expert v.13, a model was driven for the abiotic side to obtain optimum condition. The pH value was found to be 8.2 and the sulfide concentration to 2.5E-05 M. Thioalkalivibrio versutus cultivation under identified abiotic conditions resulted in biological removal of sulfide up to 1.5 g/h. The culture was not able to remove 2 g/h input sulfide, and to increase this, the biotic side should be studied.
硫化氢（HS）是一种剧毒物质，燃烧时会产生 SO 气体。因此，应将其从气流中去除。生物脱硫是去除硫化物的一种发展方法。气体生物脱硫必须加快速度，才能与化学工艺竞争。这个过程有两个方面：生物和非生物方面。为了提高硫化物去除率，应将该物质以最大量（Max.-R）提供给细菌。因此，有必要将硫化物与生物硫和氧气以及硫代硫酸盐生成的不利非生物反应速率（Min.-R）降至最低。在去微生物化的培养基中评估了最小化硫化物与生物硫和氧气以及硫代硫酸盐生成的反应速率（Min.-R）。结论是应尽可能保持低 pH 值。硫化物氧化形成硫代硫酸盐的动力学（-R）强烈依赖于硫化物浓度，为了最小化该反应速率，应缓慢将硫化物注入培养物中。为了将硫化物还原为硫化氢（Min.-R）最小化，应尽可能保持高 pH 值。使用 Design Expert v.13，为非生物侧驱动模型以获得最佳条件。发现 pH 值为 8.2，硫化物浓度为 2.5E-05 M。在确定的非生物条件下培养硫代碱杆菌 versutus 可将硫化物的生物去除率提高到 1.5 g/h。该培养物无法去除 2 g/h 的输入硫化物，为了增加这个去除量，应研究生物侧。
