School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Qingdao Central Hospital, Qingdao, China.
J Appl Microbiol. 2022 Aug;133(2):503-514. doi: 10.1111/jam.15576. Epub 2022 Apr 27.
The intestinal microbiota contributes to infant's intestine homeostasis. This study aimed to analyse how probiotics derived from breast milk promote infant intestinal development in rat pups.
The effect of potential probiotics derived from breast milk on development of intrauterine growth retardation (IUGR) newborn rats' intestine was investigated. Limosilactobacillus oris ML-329 and Lacticaseibacillus paracasei ML-446 exhibited good hydrophobicity percentages (p < 0.05). ML-446 showed a significant effect on intestinal length and weight (p < 0.05). Meanwhile, the villus height of the IUGR newborn rats fed with ML-329 was significantly higher compared with those fed with Lacticaseibacillus rhamnosus GG (p < 0.05). Moreover, ML-329 and ML-446 both significantly stimulated the proliferation and differentiation of intestinal epithelial cells by increasing the number of ki67-positive cells, goblet cells, and lysozyme-positive Paneth cells (p < 0.05) through Wnt and Notch pathway.
The proliferation and differentiation stimulating effects of ML-329 and ML-446 on IECs in the jejunum, ileum, and colon were mediated by activating the Wnt pathway with increased expression of wnt, lrp5, and β-catenin genes and accumulation of β-catenin, and by downregulating the Notch signalling pathway with decreased expression of the activated notch protein.
Lactobacillus could facilitate IUGR rat pups' intestinal development and enhance the proliferation of Paneth cells and goblet cells. These findings provide further insights into promotion of the intestinal development by breast milk-derived beneficial microbes in early life of the IUGR newborn rats.
肠道微生物群有助于婴儿的肠道稳态。本研究旨在分析来自母乳的益生菌如何促进大鼠幼仔的肠道发育。
研究了来自母乳的潜在益生菌对宫内发育迟缓（IUGR）新生大鼠肠道发育的影响。乳杆菌 ML-329 和副干酪乳杆菌 ML-446 表现出良好的疏水性百分比（p<0.05）。ML-446 对肠长度和重量有显著影响（p<0.05）。同时，与鼠李糖乳杆菌 GG 相比，喂食 ML-329 的 IUGR 新生大鼠的绒毛高度显著更高（p<0.05）。此外，ML-329 和 ML-446 均通过增加 ki67 阳性细胞、杯状细胞和溶菌酶阳性潘氏细胞的数量，显著刺激肠上皮细胞的增殖和分化（p<0.05），通过 Wnt 和 Notch 通路。
ML-329 和 ML-446 对空肠、回肠和结肠 IECs 的增殖和分化刺激作用是通过激活 Wnt 通路介导的，其特征是 wnt、lrp5 和 β-catenin 基因表达增加，β-catenin 积累，以及通过下调 Notch 信号通路介导的，表现为激活的 Notch 蛋白表达减少。
乳杆菌可促进 IUGR 大鼠幼仔的肠道发育，增强潘氏细胞和杯状细胞的增殖。这些发现为母乳来源的有益微生物在 IUGR 新生大鼠生命早期促进肠道发育提供了进一步的见解。
