Hoxie J A, Fitzharris T P, Youngbar P R, Matthews D M, Rackowski J L, Radka S F
Hum Immunol. 1987 Jan;18(1):39-52. doi: 10.1016/0198-8859(87)90111-x.
Retroviruses are known to incorporate cellular antigens as they bud from infected cells. To identify the cellular antigens that associate with the AIDS-retrovirus, we evaluated a preparation of HTLV-III antigens with a panel of monoclonal antibodies reactive with a variety of antigens expressed on the H9 T-cell line used to produce the virus. Only monoclonal antibodies that identified HLA class-II antigens, beta-2 microglobulin, and a single anti-HLA class-I antibody were reactive in an ELISA of solubilized HTLV-III virus. No reactivity was seen with 11 monoclonal antibodies to T-cell antigens or with five antibodies to determinants on HLA class-I A or B molecules. These data suggest that on H9 cells the association of budding HTLV-III virions with cellular antigens may be a nonrandom process in which some HLA antigens, particularly class-II antigens, are selectively incorporated into the viral envelope. It is possible that a selective association of HLA class II antigens with budding HTLV-III virions may also occur for T cells infected in vivo, and could have relevance for the pathogenesis of this virus.
已知逆转录病毒在从受感染细胞出芽时会整合细胞抗原。为了鉴定与艾滋病逆转录病毒相关的细胞抗原，我们用一组单克隆抗体评估了HTLV - III抗原制剂，这些单克隆抗体可与用于生产该病毒的H9 T细胞系上表达的多种抗原发生反应。在溶解的HTLV - III病毒的ELISA检测中，只有识别HLA II类抗原、β2微球蛋白的单克隆抗体以及一种抗HLA I类抗体具有反应性。针对T细胞抗原的11种单克隆抗体以及针对HLA I类A或B分子上决定簇的5种抗体均未出现反应性。这些数据表明，在H9细胞上，出芽的HTLV - III病毒颗粒与细胞抗原的结合可能是一个非随机过程，其中一些HLA抗原，特别是II类抗原，被选择性地整合到病毒包膜中。对于体内感染的T细胞，HLA II类抗原与出芽的HTLV - III病毒颗粒之间也可能发生选择性结合，这可能与该病毒的发病机制有关。
