Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada.
Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada.
Sci Rep. 2023 Dec 27;13(1):23025. doi: 10.1038/s41598-023-50365-0.
While numerous cellular proteins in the HIV envelope are known to alter virus infection, methodology to rapidly phenotype the virion surface in a high throughput, single virion manner is lacking. Thus, many human proteins may exist on the virion surface that remain undescribed. Herein, we developed a novel flow virometry screening assay to discover new proteins on the surface of HIV particles. By screening a CD4 T cell line and its progeny virions, along with four HIV isolates produced in primary cells, we discovered 59 new candidate proteins in the HIV envelope that were consistently detected across diverse HIV isolates. Among these discoveries, CD38, CD97, and CD278 were consistently present at high levels on virions when using orthogonal techniques to corroborate flow virometry results. This study yields new discoveries about virus biology and demonstrates the utility and feasibility of a novel flow virometry assay to phenotype individual virions.
虽然已知 HIV 包膜中的许多细胞蛋白会改变病毒感染，但缺乏一种快速、高通量、单个病毒的方法来表型病毒表面。因此，可能有许多人类蛋白存在于病毒表面，但尚未被描述。在此，我们开发了一种新的流病毒检测筛选测定法来发现 HIV 颗粒表面的新蛋白。通过筛选 CD4 T 细胞系及其后代病毒，以及在原代细胞中产生的四种 HIV 分离物，我们在 HIV 包膜中发现了 59 种新的候选蛋白，这些蛋白在不同的 HIV 分离物中均能被一致检测到。在这些发现中，当使用正交技术来证实流病毒检测结果时，CD38、CD97 和 CD278 始终以高水平存在于病毒颗粒上。这项研究提供了关于病毒生物学的新发现，并展示了一种新型流病毒检测测定法用于表型单个病毒的实用性和可行性。
