Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Pharm Biomed Anal. 2022 Jun 5;215:114761. doi: 10.1016/j.jpba.2022.114761. Epub 2022 Apr 11.
In this study, a fluorometric probe based on luminol-terbium coordination polymer nanoparticles (luminol-Tb CP NPs) is validated for the quantification of phenobarbital in the exhaled breath condensate collected from expiratory circuit of the mechanical ventilator (MVEBC). It relies on the coordination of phenobarbital to luminol-Tb CP NPs which casing an aggregation-induced fluorescence enhancement of probe owing to the increasing the system rigidity and the decreasing the nonradiative decay rate. As the increase in response intensity is proportional to phenobarbital amount, a method is offered for its determination in MVEBC. This method presents a linear relationship with phenobarbital concentration in the range of 0.1-10.0 mg.L with a limit of detection of 0.024 mg.L and the relative standard deviation of 3.6%. The proposed method is used in MVEBC of the pre-term newborn babies receiving phenobarbital under mechanical ventilator.
在这项研究中，验证了一种基于鲁米诺-铽配位聚合物纳米粒子（luminol-Tb CP NPs）的荧光探针，用于定量分析从呼吸机呼气回路中收集的呼出气冷凝物（MVEBC）中的苯巴比妥。它依赖于苯巴比妥与 luminol-Tb CP NPs 的配位，由于增加了系统的刚性和降低了非辐射衰减率，导致探针的聚集诱导荧光增强。由于响应强度的增加与苯巴比妥的量成正比，因此提供了一种用于在 MVEBC 中测定苯巴比妥的方法。该方法在 0.1-10.0 mg·L 范围内与苯巴比妥浓度呈线性关系，检测限为 0.024 mg·L，相对标准偏差为 3.6%。该方法用于接受机械通气的早产儿的 MVEBC 中苯巴比妥的测定。
