基于铜纳米簇的传感器用于测定呼出气冷凝物中的万古霉素：同步荧光光谱法。
Copper nanocluster-based sensor for determination of vancomycin in exhaled breath condensate: A synchronous fluorescence spectroscopy.
机构信息
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran; Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran.
Tuberculosis and Lung Diseases Research Center and Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran.
出版信息
J Pharm Biomed Anal. 2021 Mar 20;196:113906. doi: 10.1016/j.jpba.2021.113906. Epub 2021 Jan 16.
A synchronous fluorescence spectroscopy (SFS) nanoprobe is developed for the determination of vancomycin in exhaled breath condensate (EBC) samples. The synthesized nanoprobe is copper nanoclusters (Cu NCs) and its SFS peak is located at 405 nm with Δλ = 80. The affinity of Cu NCs to complex formation with vancomycin results in blocking non-radiative e/h recombination defect sites on the surface of NCs and consequently enhancing the SFS signal intensity. Central composite design and response surface methodology is used for the optimization of reaction conditions. Under the optimized conditions, a linear relationship is found between the SFS intensity and the concentration of vancomycin in the range of 0.1-8 μg/mL. The validated method is applied for the determination of vancomycin in EBC of newborns receiving vancomycin treatment.
建立了一种用于检测呼气冷凝液（EBC）中万古霉素的同步荧光光谱（SFS）纳米探针。所合成的纳米探针为铜纳米簇（Cu NCs），其 SFS 峰位于 405nm，Δλ=80nm。Cu NCs 与万古霉素形成复合物的亲和力导致在 NCs 表面阻断非辐射 e/h 复合缺陷位点，从而增强 SFS 信号强度。采用中心组合设计和响应面法优化反应条件。在优化条件下，在 0.1-8μg/mL 范围内，SFS 强度与万古霉素浓度之间存在线性关系。该方法已用于检测接受万古霉素治疗的新生儿的 EBC 中的万古霉素。
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