Carrero-Ferrer I, Molins-Legua C, Campíns-Falcó P
MINTOTA Research Group, Departament de Química Analítica, Facultat de Química. Universitat de València. Dr. Moliner 50, 46100, Burjassot, València, Spain.
MINTOTA Research Group, Departament de Química Analítica, Facultat de Química. Universitat de València. Dr. Moliner 50, 46100, Burjassot, València, Spain.
Talanta. 2022 Aug 1;245:123449. doi: 10.1016/j.talanta.2022.123449. Epub 2022 Apr 5.
In recent years, there is a growing demand for optical sensors given their analytical properties, and the possibility of in situ implementation. Among all the types of optical sensors, plasmonic sensors have aroused great interest in the scientific community. In this work, the ability of a plasmonic sensor based on AgNPs retained on a Nylon surface is studied to determine hydrogen sulfide, which can be an indicator of oral diseases. This compound produces a color change of the sensor from yellow to brown directly related to its concentration. The sensor response is evaluated in two different assay formats such as bag and well microplates. The figures of merits of both methodologies have been obtained and compared. The advantages and disadvantages of the different formats have been shown. Finally, the sensor is applied to quantify sulfides in real saliva. Concentrations ranged from 30 μ L to 600 μ L have been obtained for the voluntaries. Besides that, in this approach the RGB coordinates from images have been used as analytical signal too. The results achieved have demonstrated that the sensor and the methodology applied provide good selectivity, sensibility, rapidity, it is non-invasive and it can be used as indirect method to measure problems in the oral cavity.
近年来，鉴于其分析特性以及原位实施的可能性，对光学传感器的需求日益增长。在所有类型的光学传感器中，等离子体传感器引起了科学界的极大兴趣。在这项工作中，研究了基于保留在尼龙表面的AgNP的等离子体传感器测定硫化氢的能力，硫化氢可能是口腔疾病的一个指标。该化合物会使传感器颜色从黄色变为棕色，且颜色变化与其浓度直接相关。传感器响应在两种不同的检测形式（如袋式和微孔板）中进行评估。已获得并比较了两种方法的性能指标。展示了不同形式的优缺点。最后，该传感器应用于定量分析真实唾液中的硫化物。志愿者的唾液浓度范围为30μL至600μL。除此之外，在这种方法中，图像的RGB坐标也被用作分析信号。所取得的结果表明，所应用的传感器和方法具有良好的选择性、灵敏度、快速性，是非侵入性的，并且可以用作测量口腔问题的间接方法。
