Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, 31982, Saudi Arabia; Pharmacology Department, Zagazig University, Zagazig, 44519, Egypt.
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, 31982, Saudi Arabia; Department of Pharmacognosy, College of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.
Chem Biol Interact. 2022 Jun 1;360:109947. doi: 10.1016/j.cbi.2022.109947. Epub 2022 Apr 14.
Exploring new drugs for the management of myocardial infarction (MI) is crucial, as MI is a major contributor to mortality worldwide. Anethole, a naturally occurring essential oil component, has numerous medicinal, pharmaceutical, and cosmetic purposes. This study explored the potential action of anethole to protect myocytes against MI injure.
Wistar rats were divided into five groups: normal; anethole; and isoproterenol (ISO) groups in addition to two groups of ISO + anethole (125 and 250 mg/kg). All anethole groups were administered the oil component for 30 days, and all ISO groups were challenged with ISO on the 28th and 29th days. Parameters measured included infracted area, ECG, cardiac markers, the expression of Keap 1, nuclear Nrf2, and heme oxygenase-1, as well as the expression of TLR4 and MYD88 together with subsequent downstream oxidative stress, inflammatory, and apoptotic markers.
Anethole reduced infarct region, degenerated cardiac indicators levels, amended ECG alterations, and diminished myocardial necrosis. Anethole reduced Keap-1, activated Nrf2/HO-1 pathway, increased mitochondrial antioxidant enzyme activities, declined the TLR4/MYD88 pathway, and ameliorated myocardial inflammation and cell death markers.
Anethole may retain a cardio-protective potential by controlling myocardial oxidative stress (through Nrf2 pathway) and diminishing inflammation and apoptosis via the TLR4/MYD88 pathway.
探索用于心肌梗死（MI）管理的新药至关重要，因为 MI 是全球死亡的主要原因。茴香脑是一种天然存在的精油成分，具有许多药用、药物和化妆品用途。本研究探讨了茴香脑保护心肌细胞免受 MI 损伤的潜在作用。
Wistar 大鼠分为五组：正常组；茴香脑组；异丙肾上腺素（ISO）组，以及 ISO+茴香脑（125 和 250mg/kg）两组。所有茴香脑组均给药 30 天，所有 ISO 组在第 28 天和第 29 天接受 ISO 挑战。测量的参数包括梗死面积、心电图、心脏标志物、Keap 1 的表达、核 Nrf2 和血红素加氧酶-1，以及 TLR4 和 MYD88 及其随后的下游氧化应激、炎症和细胞凋亡标志物的表达。
茴香脑减少梗死区域、退化的心脏指标水平、纠正心电图改变、减少心肌坏死。茴香脑降低 Keap-1，激活 Nrf2/HO-1 通路，增加线粒体抗氧化酶活性，降低 TLR4/MYD88 通路，改善心肌炎症和细胞死亡标志物。
茴香脑可能通过控制心肌氧化应激（通过 Nrf2 通路）和减少炎症和细胞凋亡（通过 TLR4/MYD88 通路）来保留心脏保护潜力。
