Linde S, Welinder B S, Hansen B, Sonne O
J Chromatogr. 1986 Nov 21;369(2):327-39. doi: 10.1016/s0021-9673(00)90139-0.
Insulin monoiodinated in Tyr A14, A19, B16 and B26 can be separated from insulin and diiodoinsulins using reversed-phase high-performance liquid chromatography on LiChrosorb RP-18 columns. Monoiodoinsulins with high and low specific activities were isolated from a number of buffer systems without any reduction in binding affinity and biological activity in isolated rat fat cells. The reason for the previously observed reduction in the binding affinity was probably column bleeding, i.e., chemical degradation of the column support.
在酪氨酸A14、A19、B16和B26处单碘化的胰岛素，可使用LiChrosorb RP - 18柱上的反相高效液相色谱法与胰岛素及二碘胰岛素分离。从多种缓冲系统中分离出了具有高比活性和低比活性的单碘化胰岛素，且在分离的大鼠脂肪细胞中其结合亲和力和生物活性均未降低。之前观察到的结合亲和力降低的原因可能是柱流失，即柱填料的化学降解。
