Welinder B S, Linde S, Hansen B
J Chromatogr. 1983 Aug 19;265(2):301-9. doi: 10.1016/s0021-9673(01)96725-1.
Baseline separation between insulin and insulin monoiodinated in Tyr A14, A19, B16 and B26 can be obtained using isocratic elution from a C18 column with triethylammonium trifluoroacetate-acetonitrile and the iodinated insulin derivatives can be isolated by lyophilization. Compared with similar tracers purified and isolated by disc electrophoresis/ion-exchange chromatography, the reversed-phase high-performance liquid chromatographically purified tracers are more homogeneous but show reduced binding affinity to adipocytes.
使用C18柱，以三乙胺三氟乙酸盐 - 乙腈进行等度洗脱，可实现胰岛素与在酪氨酸A14、A19、B16和B26处单碘化的胰岛素之间的基线分离，并且碘化胰岛素衍生物可通过冻干进行分离。与通过圆盘电泳/离子交换色谱法纯化和分离的类似示踪剂相比，反相高效液相色谱法纯化的示踪剂更均匀，但对脂肪细胞的结合亲和力降低。
