通过反相高效液相色谱法（HPLC）分离不同物种的生物变异胰岛素分子。

Separation of biological variant insulin molecules from different species by reversed-phase high-performance liquid chromatography (HPLC).

作者信息

Kalant D, Crawhall J C, Posner B I

出版信息

Biochem Med. 1985 Oct;34(2):230-40. doi: 10.1016/0006-2944(85)90116-4.

Abstract

Three different isocratic systems for the separation by reversed-phase high-performance liquid chromatography (HPLC) of different species of insulin have been investigated. The effect of different solvent compositions and temperatures on elution time and resolution have been studied. These studies have been used to devise a method for reversed-phase liquid chromatographic separation of bovine, porcine, and human insulin, as well as the A and B chains of bovine insulin. The method can also be used for the separation of the various products of the iodination of porcine insulin. 125I-A14 tyrosine-labeled porcine insulin can be readily separated from nonlabeled porcine insulin and from other iodinated constituents of the mixture. A flow-though gamma-counting system that was designed for this work is described.

摘要

研究了三种不同的等度洗脱系统，用于通过反相高效液相色谱法（HPLC）分离不同种类的胰岛素。研究了不同溶剂组成和温度对洗脱时间和分离度的影响。这些研究已被用于设计一种反相液相色谱法，用于分离牛胰岛素、猪胰岛素和人胰岛素，以及牛胰岛素的A链和B链。该方法还可用于分离猪胰岛素碘化的各种产物。125I-A14酪氨酸标记的猪胰岛素可以很容易地与未标记的猪胰岛素以及混合物中的其他碘化成分分离。本文描述了为此项工作设计的一种流通式γ计数系统。