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组织蛋白酶D在正常和肿瘤性人体组织中的免疫定位

Immunolocalization of cathepsin D in normal and neoplastic human tissues.

作者信息

Reid W A, Valler M J, Kay J

出版信息

J Clin Pathol. 1986 Dec;39(12):1323-30. doi: 10.1136/jcp.39.12.1323.

DOI:10.1136/jcp.39.12.1323
PMID:3543065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1140796/
Abstract

The aspartic proteinase cathepsin D was purified from human spleen and localised in various formalin fixed paraffin embedded human tissues using the peroxidase-antiperoxidase (PAP) technique. Cathepsin D was shown not only in macrophages but also in other connective tissue cells, and in epithelium. It was present in spleen (littoral cells and cells within Malpighian bodies), liver (hepatocytes and Kupffer cells), lung (alveolar macrophages and bronchial epithelium), brain (neurones), lymph nodes (histiocytes in germinal centres, sinusoid lining cells) and stomach (parietal and mucous neck cells). Cathepsin D was also found in carcinomas of bronchus, stomach, colon, kidney, breast, ovary, bladder and pancreas, both in neoplastic epithelium and in stromal cells, but was seldom present in connective tissue neoplasms. A group of malignant lymphomas also contained the enzyme within scattered cells. The distribution of cathepsin D seems to be much wider than that of the structurally related aspartic proteinases pepsin, gastricsin, and renin.

摘要

天冬氨酸蛋白酶组织蛋白酶D从人脾脏中纯化出来，并使用过氧化物酶-抗过氧化物酶（PAP）技术定位在各种福尔马林固定石蜡包埋的人体组织中。组织蛋白酶D不仅在巨噬细胞中出现，还在其他结缔组织细胞和上皮细胞中出现。它存在于脾脏（边缘细胞和马氏体中的细胞）、肝脏（肝细胞和库普弗细胞）、肺（肺泡巨噬细胞和支气管上皮）、脑（神经元）、淋巴结（生发中心的组织细胞、血窦衬里细胞）和胃（壁细胞和黏液颈细胞）中。组织蛋白酶D也在支气管、胃、结肠、肾、乳腺、卵巢、膀胱和胰腺的癌组织中被发现，存在于肿瘤上皮细胞和基质细胞中，但很少出现在结缔组织肿瘤中。一组恶性淋巴瘤的散在细胞中也含有这种酶。组织蛋白酶D的分布似乎比结构相关的天冬氨酸蛋白酶胃蛋白酶、胃泌酶和肾素的分布要广泛得多。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/db17ec3e1df2/jclinpath00320-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/21f935edc541/jclinpath00320-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/bd9002bbe470/jclinpath00320-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/f07fcf49366f/jclinpath00320-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/3d57c430bfbc/jclinpath00320-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/a67f26ea8140/jclinpath00320-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/f1d22145cb59/jclinpath00320-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/71797f894548/jclinpath00320-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/2d5052c0baf6/jclinpath00320-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/db17ec3e1df2/jclinpath00320-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/21f935edc541/jclinpath00320-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/bd9002bbe470/jclinpath00320-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/f07fcf49366f/jclinpath00320-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/3d57c430bfbc/jclinpath00320-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/a67f26ea8140/jclinpath00320-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/f1d22145cb59/jclinpath00320-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/71797f894548/jclinpath00320-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/2d5052c0baf6/jclinpath00320-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8369/1140796/db17ec3e1df2/jclinpath00320-0049-a.jpg

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2
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Biochem J. 1981 Aug 1;197(2):519-22. doi: 10.1042/bj1970519.
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Brain renin.脑肾素
高表达溶酶体蛋白酶组织蛋白酶 D 通过对比 EGF 诱导的神经母细胞瘤细胞生长来改善神经母细胞瘤患者的预后。
Int J Mol Sci. 2022 Apr 26;23(9):4782. doi: 10.3390/ijms23094782.
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Differential role of melatonin in healthy brain aging: a systematic review and meta-analysis of the SAMP8 model.褪黑素在健康大脑衰老中的差异作用：SAM-P8 模型的系统评价和荟萃分析。
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Development of new in vitro models of lung protease activity for investigating stability of inhaled biological therapies and drug delivery systems.开发新型体外肺蛋白酶活性模型，以研究吸入性生物疗法和药物输送系统的稳定性。
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Cathepsin D deficiency delays central nervous system myelination by inhibiting proteolipid protein trafficking from late endosome/lysosome to plasma membrane.组织蛋白酶 D 缺乏通过抑制少突胶质细胞髓鞘蛋白从晚期内体/溶酶体向质膜的转运从而延迟中枢神经系统髓鞘形成。
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