Robson D K, Ironside J W, Reid W A, Bogue P R
Department of Pathology, University of Leeds.
Neuropathol Appl Neurobiol. 1990 Feb;16(1):39-44. doi: 10.1111/j.1365-2990.1990.tb00930.x.
The cellular distribution of the lysosomal proteinase cathepsin D was studied in a series of 76 neoplasms and 18 non-neoplastic tissues from the human central nervous system, using a well-characterized polyclonal antibody in a peroxidase-antiperoxidase technique. In the normal and developing brain, cathepsin D is confined to neurons and choroid plexus epithelium. Strong granular cytoplasmic staining was present in neuronal and choroid plexus neoplasms, and in reactive macrophages. A large variety of other neoplasms also exhibited positive cytoplasmic staining, albeit usually of a weaker diffuse type. Cathepsin D cannot be considered a specific marker for neuronal or choroid plexus neoplasms, but the antiserum used in this study may be of value in antibody panels for the investigation of these tumours. Its localization may also be of value in embryological studies, particularly in the cerebellum, and in investigations of steroid hormone receptor-associated proteins in meningiomas and Schwannomas.
运用过氧化物酶-抗过氧化物酶技术，使用一种特性明确的多克隆抗体，对取自人类中枢神经系统的76例肿瘤和18例非肿瘤组织进行研究，以观察溶酶体蛋白酶组织蛋白酶D的细胞分布情况。在正常及发育中的脑内，组织蛋白酶D局限于神经元和脉络丛上皮细胞。在神经元肿瘤、脉络丛肿瘤以及反应性巨噬细胞中，可见强颗粒状胞质染色。多种其他肿瘤也呈现胞质阳性染色，不过通常为较弱的弥漫型。组织蛋白酶D不能被视为神经元或脉络丛肿瘤的特异性标志物，但本研究中使用的抗血清在用于这些肿瘤研究的抗体组合中可能具有价值。其定位在胚胎学研究中，尤其是在小脑的研究中，以及在脑膜瘤和神经鞘瘤中类固醇激素受体相关蛋白的研究中，可能也具有价值。
