Nestor M S, Cochran A J, Ahmed A R
J Invest Dermatol. 1987 Feb;88(2):172-5. doi: 10.1111/1523-1747.ep12525315.
Mononuclear cell subset-specific monoclonal antibodies, indirect immunoperoxidase labeling, and a specific quantification technique were used to determine the constitution of mononuclear cell infiltrates associated with bullous pemphigoid (BP), pemphigus vulgaris (PV), dermatitis herpetiformis (DH), and linear IgA bullous disease, as well as those in normal skin (NS) from healthy adult volunteers. Bullous pemphigoid and PV lesions contain significantly greater numbers of T lymphocytes (p less than 0.001), including both T helper/inducer and T cytotoxic/suppressor subsets, macrophages (p less than 0.002), and Langerhans cells (p less than 0.01), than NS or lesions of DH, supporting the concept that cell-mediated immunity is involved in bulla formation and autoantigen presentation in BP and PV. The infiltrates associated with DH lesions appear to reflect a different mechanism of autoantibody production and bulla formation.
采用单核细胞亚群特异性单克隆抗体、间接免疫过氧化物酶标记及特定定量技术，以确定与大疱性类天疱疮（BP）、寻常型天疱疮（PV）、疱疹样皮炎（DH）及线状IgA大疱病相关的单核细胞浸润的组成，以及来自健康成年志愿者正常皮肤（NS）中的单核细胞浸润组成。与NS或DH皮损相比，BP和PV皮损含有显著更多的T淋巴细胞（p<0.001），包括T辅助/诱导亚群和T细胞毒性/抑制亚群、巨噬细胞（p<0.002）和朗格汉斯细胞（p<0.01），这支持了细胞介导的免疫参与BP和PV中水疱形成及自身抗原呈递的观点。与DH皮损相关的浸润似乎反映了自身抗体产生和水疱形成的不同机制。
