Prensier G, Slomianny C
J Parasitol. 1986 Oct;72(5):731-6.
The karyotype of the erythrocytic stages of Plasmodium falciparum was determined to be 14 by counting the number of kinetochores in the mitotic spindle of young schizonts. Fourteen pairs of kinetochores were identified in 3-dimensional reconstructions of the spindle derived from serial longitudinal and transverse sections. Kinetochores were ovoid (45 X 25 nm) in transverse sections, measured 100 nm in longitudinal section, and were heptalaminate in structure. A pair of spindle microtubules passed through each kinetochore. In schizonts at anaphase, each pole of the mitotic spindle consisted of paired kinetic centers inserted on the nuclear membrane. The kinetic centers resembled nuclear pores, but were more electron dense and were associated with spherical masses of an electron-dense cytoplasmic material.
通过计算年轻裂殖体有丝分裂纺锤体中的动粒数量，确定恶性疟原虫红细胞阶段的核型为14。在由连续纵向和横向切片获得的纺锤体三维重建中，鉴定出14对动粒。动粒在横切面上呈椭圆形（45×25纳米），在纵切面上测量为100纳米，结构为七层。一对纺锤体微管穿过每个动粒。在后期裂殖体中，有丝分裂纺锤体的每个极由插入核膜的成对动力中心组成。动力中心类似于核孔，但电子密度更高，并与电子致密细胞质物质的球形团块相关联。
