Witkin S S, Zelikovsky G
J Clin Lab Immunol. 1986 Sep;21(1):7-10.
16 men with chronic prostatitis were evaluated immunologically in order to examine possible relationships between prostate infection, defective cellular immune responses and the occurrence of sperm antibodies. Peripheral blood mononuclear cells (PBMN) from 14 of 16 (88%) patients exhibited reduced or absent responses in vitro when incubated with an extract of Candida albicans. PBMN proliferation in response to the mitogen Concanavalin A (Con A) was reduced in 5 of 16 (31%) men. In addition, sera from 6 patients inhibited the Candida-induced proliferative response of control PBMC; 2 of these sera also reduced the Con A-directed response. Sperm antibodies, evaluated by an enzyme-linked immunosorbent assay (ELISA) using fresh motile spermatozoa, were found in 9 of 16 (56%) patients. PBMC responses were reduced in all, and suppressive sera present in 5, of the men with sperm antibodies. IgG antibodies predominated. 4 of the sera positive by ELISA also agglutinated spermatozoa. Levels of IgG sperm antibodies were correlated with the degree of immunosuppression by patient sera (p less than 0.02). These data suggest that decreased cellular immunity and enhanced humoral reactivity to sperm are common in men with chronic prostatitis. Both may contribute to an increased rate of prostatic infection in these men.
对16名慢性前列腺炎患者进行了免疫学评估，以研究前列腺感染、细胞免疫反应缺陷与精子抗体产生之间可能存在的关系。16名患者中有14名（88%）的外周血单核细胞（PBMN）在与白色念珠菌提取物孵育时，体外反应减弱或缺失。16名男性中有5名（31%）对促有丝分裂原刀豆球蛋白A（Con A）的PBMN增殖反应降低。此外，6名患者的血清抑制了对照PBMC的念珠菌诱导的增殖反应；其中2份血清也降低了Con A诱导的反应。通过使用新鲜活动精子的酶联免疫吸附测定（ELISA）评估，16名患者中有9名（56%）发现了精子抗体。所有有精子抗体的男性的PBMC反应均降低，其中5名存在抑制性血清。IgG抗体占主导。ELISA检测呈阳性的4份血清也使精子发生凝集。IgG精子抗体水平与患者血清的免疫抑制程度相关（p<0.02）。这些数据表明，细胞免疫降低和对精子的体液反应性增强在慢性前列腺炎男性中很常见。两者都可能导致这些男性前列腺感染率增加。
