Alexander R B, Brady F, Ponniah S
Urology Section, VA Maryland Health Care System, Baltimore 21201, USA.
Urology. 1997 Dec;50(6):893-9. doi: 10.1016/S0090-4295(97)00456-1.
To determine whether men with chronic prostatitis/chronic pelvic pain syndrome have evidence of an autoimmune response to prostatic proteins. We examined men with a history of chronic prostatitis/ chronic pelvic pain syndrome for evidence of T lymphocyte reactivity to seminal plasma.
Patients underwent automated leukopheresis to obtain peripheral blood mononuclear cells. We performed a recall antigen proliferation assay to detect specific proliferation of peripheral helper T lymphocytes in men. with chronic prostatitis/chronic pelvic pain syndrome and compared the results with those of normal men. The antigen for these studies consisted of seminal plasma from normal donors and men with seminal vesicle atresia.
A specific recall proliferative response to seminal plasma was observed in 3 of 10 men with a history of chronic prostatitis/chronic pelvic pain syndrome compared with none of 15 normal men. The CD4 T cell proliferative response to seminal plasma was statistically significant when compared with medium alone in men with a history of chronic prostatitis/chronic pelvic pain syndrome but it was not statistically significant in normal men. The recall responses of both the chronic prostatitis/chronic pelvic pain syndrome group and normal subjects to the recall antigens tetanus toxoid and Candida extract were equivalent.
The data represent the first direct evidence that some men with chronic prostatitis/chronic pelvic pain syndrome have an autoimmune component to their disease. Autoimmunity is a potential etiology for chronic nonbacterial prostatitis.
确定患有慢性前列腺炎/慢性盆腔疼痛综合征的男性是否有针对前列腺蛋白的自身免疫反应证据。我们检查了有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性，以寻找T淋巴细胞对精浆反应性的证据。
患者接受自动白细胞分离术以获取外周血单个核细胞。我们进行了回忆抗原增殖试验，以检测患有慢性前列腺炎/慢性盆腔疼痛综合征男性外周辅助性T淋巴细胞的特异性增殖，并将结果与正常男性的结果进行比较。这些研究的抗原包括正常供体和患有精囊闭锁男性的精浆。
10名有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性中有3名观察到对精浆的特异性回忆增殖反应，而15名正常男性中无一例出现此反应。与单独使用培养基相比，有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性中，CD4 T细胞对精浆的增殖反应具有统计学意义，但在正常男性中无统计学意义。慢性前列腺炎/慢性盆腔疼痛综合征组和正常受试者对破伤风类毒素和念珠菌提取物等回忆抗原的反应相当。
这些数据首次直接证明，一些患有慢性前列腺炎/慢性盆腔疼痛综合征的男性其疾病存在自身免疫成分。自身免疫是慢性非细菌性前列腺炎的一个潜在病因。
