Association of selected polymorphisms in and genes in Iranian women with idiopathic recurrent pregnancy loss: A case-control study.

BACKGROUND

Recurrent pregnancy loss (RPL) is a major concern among women worldwide. However, the exact mechanisms underlying miscarriage are not well understood. Recent evidence suggests that single nucleotide polymorphisms in various genes, especially miRNAs, may be responsible for RPL.

OBJECTIVE

We surveyed the association between polymorphisms in and in Iranian women with idiopathic RPL.

MATERIALS AND METHODS

DNA was extracted from blood samples of 116 women with idiopathic RPL and 89 healthy women as controls who had previously had at least two successful pregnancies. Polymerase chain reaction was used for the amplification of the genes. Genotype screening along with SNaPshot were performed to detect different polymorphisms. Finally, the polymorphisms and frequency of each genotype were compared between the two groups.

RESULTS

The frequencies of polymorphisms in (p 0.001) and (p = 0.04) were calculated among the case and control groups, which showed a statistical difference (p 0.05), indicating an association between these polymorphisms and the symptoms of RPL. The frequencies of polymorphisms of genotypes in and did not demonstrate any difference between the two groups. Also, the amount of alleles in and were significantly different (p 0.001 and p = 0.02, respectively) and the dominant inheritance model was proposed.

CONCLUSION

In conclusion, and can be associated with RPL in women. The SNaPshot technique is a valuable tool to evaluate possible associations between polymorphisms and health conditions.