Nemati Vahedi Sara, Kheirkhah Babak, Akbar Malekirad Ali, Mostafa Hosseini Sayed
Department of Cellular and Molecular Biology, Faculty of Basic Sciences, Islamic Azad University Sirjan Branch, Sirjan, Iran.
Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University Baft Branch, Baft, Iran.
Int J Reprod Biomed. 2022 Mar 21;20(2):111-122. doi: 10.18502/ijrm.v20i2.10503. eCollection 2022 Mar.
Recurrent pregnancy loss (RPL) is a major concern among women worldwide. However, the exact mechanisms underlying miscarriage are not well understood. Recent evidence suggests that single nucleotide polymorphisms in various genes, especially miRNAs, may be responsible for RPL.
We surveyed the association between polymorphisms in and in Iranian women with idiopathic RPL.
DNA was extracted from blood samples of 116 women with idiopathic RPL and 89 healthy women as controls who had previously had at least two successful pregnancies. Polymerase chain reaction was used for the amplification of the genes. Genotype screening along with SNaPshot were performed to detect different polymorphisms. Finally, the polymorphisms and frequency of each genotype were compared between the two groups.
The frequencies of polymorphisms in (p 0.001) and (p = 0.04) were calculated among the case and control groups, which showed a statistical difference (p 0.05), indicating an association between these polymorphisms and the symptoms of RPL. The frequencies of polymorphisms of genotypes in and did not demonstrate any difference between the two groups. Also, the amount of alleles in and were significantly different (p 0.001 and p = 0.02, respectively) and the dominant inheritance model was proposed.
In conclusion, and can be associated with RPL in women. The SNaPshot technique is a valuable tool to evaluate possible associations between polymorphisms and health conditions.
复发性流产（RPL）是全球女性关注的主要问题。然而，流产的确切机制尚未完全了解。最近的证据表明，各种基因中的单核苷酸多态性，尤其是微小RNA，可能与复发性流产有关。
我们调查了伊朗特发性复发性流产女性中[具体基因1]和[具体基因2]多态性之间的关联。
从116例特发性复发性流产女性和89例健康女性（作为对照，这些健康女性此前至少有两次成功妊娠）的血样中提取DNA。采用聚合酶链反应扩增基因。进行基因分型筛选并结合SNaPshot技术检测不同的多态性。最后，比较两组之间每种基因型的多态性和频率。
计算病例组和对照组中[具体基因1]（p < 0.001）和[具体基因2]（p = 0.04）多态性的频率，结果显示有统计学差异（p < 0.05），表明这些多态性与复发性流产症状之间存在关联。[具体基因1]和[具体基因2]基因型的多态性频率在两组之间未显示出任何差异。此外，[具体基因1]和[具体基因2]的等位基因数量也有显著差异（分别为p < 0.001和p = 0.02），并提出了显性遗传模型。
总之，[具体基因1]和[具体基因2]可能与女性复发性流产有关。SNaPshot技术是评估多态性与健康状况之间可能关联的有价值工具。
