Bin Zou, Feng Liu, Yan Yan
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2022 Sep 15;388:132898. doi: 10.1016/j.foodchem.2022.132898. Epub 2022 Apr 5.
This study constructed a novel biomimetic enzyme electrochemical biosensor based on tetraphenyl metalloporphyrins functionalized multi-walled carbon nanotubes (MWCNTs). The effect of central metal ions on the catalytic activity of tetraphenyl metalloporphyrin biomimetic enzyme was investigated. It was found that the change of central metal ions had a significant effect on the catalytic performance of metalloporphyrin and Zinc(II) tetraphenylporphyrin (ZnTPP) had the most excellent catalytic property. The electrochemical behaviors of tert-butylhydroquinone (TBHQ) on ZnTPP/MWCNTs modified electrode were investigated. It was found that the redox peak current was increased significantly, which was attributed to the redox peak current to the electrocatalytic activity of ZnTPP and the synergistic effect between ZnTPP and MWCNTs. A strong linear relationship was shown in the concentration range of 0.01 to 1000 μM. This electrochemical sensor also had excellent repeatability, storage, and interference resistance. This work provided a simple and sensitive method for the determination of TBHQ.
本研究构建了一种基于四苯基金属卟啉功能化多壁碳纳米管（MWCNTs）的新型仿生酶电化学生物传感器。研究了中心金属离子对四苯基金属卟啉仿生酶催化活性的影响。发现中心金属离子的变化对金属卟啉的催化性能有显著影响，其中四苯基卟啉锌（ZnTPP）具有最优异的催化性能。研究了叔丁基对苯二酚（TBHQ）在ZnTPP/MWCNTs修饰电极上的电化学行为。发现氧化还原峰电流显著增加，这归因于ZnTPP的电催化活性以及ZnTPP与MWCNTs之间的协同效应导致的氧化还原峰电流。在0.01至1000μM的浓度范围内呈现出良好的线性关系。该电化学生物传感器还具有优异的重复性、储存稳定性和抗干扰性。本工作为TBHQ的测定提供了一种简单且灵敏的方法。
