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用于酵母鉴定的商用多项检测系统的评估

Evaluation of a commercial multitest system for identification of yeasts.

作者信息

Qadri S M, Nichols C W

出版信息

Am J Med Technol. 1978 May;44(5):368-72.

PMID:354378
Abstract

A new commercial yeast identification system, the Randolph Multitest Mycology Plate, was evaluated. This system is based on the assimilation of carbohydrates, growth on birdseed agar, resistance to cycloheximide, hydrolysis of urea, and reduction of nitrate. The 184 clinical isolates used in the evaluation included 20 species of the genera Candida, Cryptococcus, Saccharomyces, Torulopsis, Trichosporon, Rhodotorula, and Geotrichum. Agreement with the standard Wickerham carbohydrate assimilation and other conventional biochemical results was found in 84 percent of the tests, and 91 percent of the yeasts were correctly identified with the aid of microscopy, cultural characteristics, and germ-tube formation. Identification of the tested strains was achieved within 96 hours.

摘要

对一种新的商业酵母鉴定系统——伦道夫多重检测真菌平板进行了评估。该系统基于碳水化合物同化、在鸟籽琼脂上生长、对放线菌酮的抗性、尿素水解及硝酸盐还原。评估中使用的184株临床分离株包括念珠菌属、隐球菌属、酿酒酵母属、球拟酵母属、丝孢酵母属、红酵母属和地霉属的20个种。84%的检测结果与标准的威克汉姆碳水化合物同化及其他传统生化结果相符,借助显微镜检查、培养特性和芽管形成,91%的酵母被正确鉴定。在96小时内完成了对受试菌株的鉴定。

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