[CHROM-agar念珠菌培养基在临床相关酵母菌鉴别及初步鉴定中的应用价值]
[Usefulness of the CHROM-agar Candida culture medium in the differentiation and presumed identification of yeast of clinical interest].
作者信息
García-Martos P, Mira-Gutiérrez J, Galán-Sánchez F, Hernández-Molina J M
机构信息
Servicio de Microbiología, Hospital Universitario Puerta del Mar, Cádiz.
出版信息
Enferm Infecc Microbiol Clin. 1997 Feb;15(2):70-2.
BACKGROUND
The efficiency of a novel differential culture medium, CHROM-agar Candida, is evaluated in order to differentiation and presumptive identification of clinically important yeasts.
METHODS
We studied 600 clinical yeast strains, pertaining to 8 genera (Candida, Cryptococcus, Geotrichum, Rhodotorula, Trichosporon, Kluyveromyces, Pichia and Saccharomyces) and 27 species. Strains were previously identified by conventional methods and by commercial system ATB ID32C (Bio-Mérieux).
RESULTS
Four species (C. albicans, C. krusei, C. tropicalis and t. beigelii) were presumptive recognized after 48-72 hours of incubation, regarding their colonial morphology and color. Colony color of other species showed low specificity.
CONCLUSION
CHROM-agar Candida medium is recommended for recognition of mixed yeast cultures and differentiation of the common clinically yeast species.
背景
为了对临床上重要的酵母进行鉴别和初步鉴定，对一种新型鉴别培养基CHROM-琼脂念珠菌培养基的效率进行了评估。
方法
我们研究了600株临床酵母菌株，它们属于8个属（念珠菌属、隐球菌属、地霉属、红酵母属、丝孢酵母属、克鲁维酵母属、毕赤酵母属和酿酒酵母属）和27个种。这些菌株先前已通过传统方法和商业系统ATB ID32C（生物梅里埃公司）进行鉴定。
结果
四种菌（白色念珠菌、克柔念珠菌、热带念珠菌和季也蒙念珠菌）在培养48 - 72小时后，根据其菌落形态和颜色被初步识别。其他菌种的菌落颜色特异性较低。
结论
推荐使用CHROM-琼脂念珠菌培养基来识别混合酵母培养物并鉴别常见的临床酵母菌种。
Zotero 插件