Neural Systems Laboratory, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Mental Health Theme, Florey Institute of Neuroscience and Mental Health, Melbourne, Australia.
Sci Data. 2022 Apr 19;9(1):175. doi: 10.1038/s41597-022-01268-8.
The dopaminergic system undergoes major reorganization during development, a period especially vulnerable to mental disorders. Forebrain neurons expressing dopamine 1 and 2 receptors (D1R and D2R, respectively) play a key role in this system. However, neuroanatomical information about the typical development of these neurons is sparse and scattered across publications investigating one or a few brain regions. We here present a public online collection of microscopic images of immunohistochemically stained serial sections from male and female mice at five stages of development (postnatal day 17 (P17), P25, P35, P49, and adult), showing the distribution of D1R and D2R expressing neurons across the forebrain. All images from adult brains are registered to the Allen Mouse brain Common Coordinate Framework, while images from P17-P35 age groups are registered to spatially modified atlas versions matching the morphology of young brains. This online resource provides microscopic visualization of the developing dopaminergic system in mice, which is suitable as a benchmark reference for performing new experiments and building computational models of the brain.
多巴胺能系统在发育过程中经历了重大的重组，这一时期特别容易受到精神障碍的影响。表达多巴胺 1 和 2 受体（分别为 D1R 和 D2R）的前脑神经元在该系统中发挥着关键作用。然而，关于这些神经元的典型发育的神经解剖学信息是稀疏的，分散在研究一个或几个脑区的出版物中。我们在这里提供了一个公开的在线收集，其中包含来自雄性和雌性小鼠五个发育阶段（出生后第 17 天（P17）、P25、P35、P49 和成年）的免疫组织化学染色连续切片的微观图像，展示了表达 D1R 和 D2R 的神经元在前脑中的分布。成年大脑的所有图像都注册到 Allen Mouse brain Common Coordinate Framework 中，而 P17-P35 年龄组的图像则注册到与年轻大脑形态匹配的空间修正图谱版本中。这个在线资源提供了小鼠发育中的多巴胺能系统的微观可视化，适合作为基准参考，用于进行新的实验和构建大脑的计算模型。
