School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China.
State Key Laboratory of Cotton Biology, Anyang, 455000, Henan, China.
Mol Biol Rep. 2022 Jul;49(7):6405-6413. doi: 10.1007/s11033-022-07449-3. Epub 2022 Apr 19.
Upland cotton is one of the main cultivated species of cotton, and salt stress is an important factor in its growth and development. Wall-associated receptor kinase galacturonan binding (GUB_WAK_bind) is an extracellular domain of wall-associated kinase (WAK), which can sense the environment and play a role in the response to plant stress.
In this study, the GUB_WAK_bind gene in Gossypium hirsutum was identified and analyzed by bioinformatics at the whole genome level, including its physicochemical properties, evolutionary development, gene structure, chromosome positioning, cis-acting elements in the promoter, etc., and the expression of the GUB_WAK_bind genes under salt stress were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). A total of 22 GUB_WAK_bind gene members were identified in Gossypium hirsutum and divided into three subgroups by evolutionary development and motif analysis, most of which contained motif 5, which is similar to the motif pattern of subgroup members. The number of exons in this gene family is between 1 and 4, the number of introns is between 0 and 3, and 22 gene members are distributed on 14 chromosomes of Gossypium hirsutum. Almost all gene members have adverse stress response elements in their promoter region. The expression analysis in response to salt stress showed that the selected six genes were induced by NaCl stress with significant expression differences (P < 0.05).
The results of this study have a certain reference value for understanding the evolution and function of GUB_WAK_bind genes and studying the salt tolerance genes of Gossypium hirsutum.
陆地棉是棉花的主要栽培种之一，盐胁迫是影响其生长发育的重要因素。壁相关受体激酶半乳糖醛酸结合（GUB_WAK_bind）是壁相关激酶（WAK）的细胞外结构域，能够感知环境并在植物应激反应中发挥作用。
本研究在全基因组水平上通过生物信息学方法鉴定和分析了陆地棉的 GUB_WAK_bind 基因，包括其理化性质、进化发育、基因结构、染色体定位、启动子中的顺式作用元件等，并通过定量实时聚合酶链反应（qRT-PCR）分析了 GUB_WAK_bind 基因在盐胁迫下的表达。在陆地棉中鉴定出 22 个 GUB_WAK_bind 基因成员，并通过进化发育和基序分析将其分为三个亚组，其中大多数亚组包含基序 5，与亚组成员的基序模式相似。该基因家族的外显子数在 1 到 4 之间，内含子数在 0 到 3 之间，22 个基因成员分布在陆地棉的 14 条染色体上。该基因家族几乎所有成员的启动子区域都含有逆境胁迫响应元件。对盐胁迫响应的表达分析表明，所选的 6 个基因均被 NaCl 胁迫诱导，表达差异显著（P<0.05）。
本研究结果对了解 GUB_WAK_bind 基因的进化和功能以及研究陆地棉的耐盐基因具有一定的参考价值。
