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长链非编码 RNA PVT1 在肾细胞癌中增加，并影响体外的活力和迁移。

LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro.

机构信息

Central European Institute of Technology, Masaryk University, Brno, Czech Republic.

Department of Urology, The University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

出版信息

J Clin Lab Anal. 2022 Jun;36(6):e24442. doi: 10.1002/jcla.24442. Epub 2022 Apr 20.

DOI:10.1002/jcla.24442
PMID:35441392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9169165/
Abstract

BACKGROUND

Renal cell carcinoma is difficult to diagnose and unpredictable in disease course and severity. There are no specific biomarkers for diagnosis and prognosis estimation feasible in clinical practice. Long non-coding RNAs (lncRNAs) have emerged as potent regulators of gene expression in recent years. Aside from their cellular role, their expression patterns could be used as a biomarker of ongoing pathology.

METHODS

In this work, we used next-generation sequencing for global lncRNA expression profiling in tumor and non-tumor tissue of RCC patients. The four candidate lncRNAs have been further validated on an independent cohort. PVT1, as the most promising lncRNA, has also been studied using functional in vitro tests.

RESULTS

Next-generation sequencing showed significant dysregulation of 1163 lncRNAs; among them top 20 dysregulated lncRNAs were AC061975.7, AC124017.1, AP000696.1, AC148477.4, LINC02437, GATA3-AS, LINC01762, LINC01230, LINC01271, LINC01187, LINC00472, AC007849.1, LINC00982, LINC01543, AL031710.1, and AC019197.1 as down-regulated lncRNAs; and SLC16A1-AS1, PVT1, LINC0887, and LUCAT1 as up-regulated lncRNAs. We observed statistically significant dysregulation of PVT1, LUCAT1, and LINC00982. Moreover, we studied the effect of artificial PVT1 decrease in renal cell line 786-0 and observed an effect on cell viability and migration.

CONCLUSION

Our results show not only the diagnostic but also the therapeutic potential of PVT1 in renal cell carcinoma.

摘要

背景

肾细胞癌的诊断较为困难，其病程和严重程度难以预测。目前尚无可行的临床诊断和预后评估的特异性生物标志物。近年来，长链非编码 RNA（lncRNA）已成为基因表达的有效调控因子。除了其在细胞中的作用外，其表达模式也可作为病理过程的生物标志物。

方法

本研究采用下一代测序技术对肾细胞癌患者肿瘤和非肿瘤组织中的 lncRNA 进行了全面表达谱分析。在另一独立队列中进一步验证了这 4 个候选 lncRNA。PVT1 作为最有前途的 lncRNA，也进行了功能体外试验研究。

结果

下一代测序显示 1163 个 lncRNA 显著失调；其中前 20 个失调的 lncRNA 是 AC061975.7、AC124017.1、AP000696.1、AC148477.4、LINC02437、GATA3-AS、LINC01762、LINC01230、LINC01271、LINC01187、LINC00472、AC007849.1、LINC00982、LINC01543、AL031710.1 和 AC019197.1，为下调的 lncRNA；而 SLC16A1-AS1、PVT1、LINC0887 和 LUCAT1 为上调的 lncRNA。我们观察到 PVT1、LUCAT1 和 LINC00982 的表达存在统计学显著差异。此外，我们研究了人工降低肾细胞系 786-0 中 PVT1 的表达对细胞活力和迁移的影响。

结论

我们的研究结果不仅显示了 PVT1 在肾细胞癌中的诊断潜力，也显示了其治疗潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/bf1d152da7a0/JCLA-36-e24442-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/236de83a5e6a/JCLA-36-e24442-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/6a6ea85f5e0c/JCLA-36-e24442-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/89dc3e77f91a/JCLA-36-e24442-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/cc0e76481823/JCLA-36-e24442-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/1b7986934ce5/JCLA-36-e24442-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/4287d572de5a/JCLA-36-e24442-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/f7ab0d5ebc23/JCLA-36-e24442-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/bf1d152da7a0/JCLA-36-e24442-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/236de83a5e6a/JCLA-36-e24442-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/6a6ea85f5e0c/JCLA-36-e24442-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/89dc3e77f91a/JCLA-36-e24442-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/cc0e76481823/JCLA-36-e24442-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/1b7986934ce5/JCLA-36-e24442-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/4287d572de5a/JCLA-36-e24442-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/f7ab0d5ebc23/JCLA-36-e24442-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b7/9169165/bf1d152da7a0/JCLA-36-e24442-g002.jpg

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2
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3
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Cancer Cell Int. 2024 Mar 30;24(1):122. doi: 10.1186/s12935-024-03285-6.
4
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