Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005315.
A Gram-stain-positive, aerobic actinobacterial strain designated MMS17-BM035 isolated from mountain soil around a decaying tree was subjected to taxonomic characterization. The isolate developed extensively branched substrate mycelia and white aerial hyphae on International Project 2 agar. Strain MMS17-BM035 grew at 15-34 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-6 % NaCl (optimum, 0 %). Analysis of 16S rRNA gene sequences indicated that MMS17-BM035 fell into a phylogenetic cluster belonging to the genus . MMS17-BM035 shared the highest sequence similarity of 99.45 % with JBL-20, and no higher than 98.7 % with other species of . Based on the orthologous average nucleotide identity, MMS17-BM035 was again mostly related to JBL-20 with 84.14 % identity, and less than 80 % with other species. The digital DNA-DNA hybridization analysis also indicated low levels of relatedness with related species, as the highest value was observed with JBL-20 (28.8 %). The major fatty acids of the strain were anteiso-C, a summed feature (consisting of C ω7/C ω6), iso-C, C and C. The major respiratory quinones were MK-9(H) and MK-9(H). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositolmannoside. The major cell-wall diamino acid was ll-diaminopimelic acid, and the characteristic whole-cell sugars were glucose and ribose. The DNA G+C content was 72.1 mol%. Strain MMS17-BM035 exhibited antimicrobial activity against several Gram-positive bacteria and yeasts. Based on both phenotypic and phylogenetic evidences, strain MMS17-BM035 should be classified as representing a novel species, for which the name sp. nov. (type strain=MMS17-BM035=KCTC 49544=JCM 34528) is proposed.
一株革兰氏阳性、需氧放线菌菌株，编号为 MMS17-BM035，从腐烂树木周围的山地土壤中分离得到，进行了分类鉴定。该菌株在国际项目 2 琼脂上产生广泛分枝的基质菌丝和白色气生菌丝。MMS17-BM035 菌株在 15-34°C（最适温度 30°C）、pH 5.0-8.0（最适 pH 7.0）和 0-6%NaCl（最适 0%）的条件下生长。16S rRNA 基因序列分析表明，MMS17-BM035 属于 属的一个进化分支。MMS17-BM035 与 JBL-20 的序列相似度最高，为 99.45%，与其他 属的物种相似度不高于 98.7%。根据直系同源平均核苷酸同一性，MMS17-BM035 再次与 JBL-20 最为相关，同源性为 84.14%，与其他物种的同源性低于 80%。数字 DNA-DNA 杂交分析也表明与相关物种的相关性较低，最高值与 JBL-20（28.8%）观察到。该菌株的主要脂肪酸为 anteiso-C、summed feature（由 C ω7/C ω6 组成）、iso-C、C 和 C。主要呼吸醌为 MK-9(H)和 MK-9(H)。诊断性极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇甘露糖苷。主要细胞壁二氨基庚二酸为 ll-二氨基庚二酸，全细胞特征性糖为葡萄糖和核糖。DNA G+C 含量为 72.1mol%。MMS17-BM035 菌株对几种革兰氏阳性细菌和酵母表现出抗菌活性。基于表型和系统发育证据，菌株 MMS17-BM035 应归类为代表一个新种，建议将其命名为 sp. nov.（模式菌株=MMS17-BM035=KCTC 49544=JCM 34528）。
