Department of Molecular Biology and Genetics, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey.
Vocational School of Health Services, Department of Medical Laboratory Techniques, Sinop University, 57000, Sinop, Turkey.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4791-4797. doi: 10.1099/ijsem.0.004347. Epub 2020 Jul 23.
A novel actinobacteria, isolate A7024, was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024 was positioned within the members of the genus with the highest sequence similarity (97.7 %) to ZFG47. The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024 contained ll-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H) and MK-9(H). The major cellular fatty acids were iso-C, anteisoC and anteiso-C. Strain A7024 had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 ± 2.3% digital DNA-DNA hybridization and 76.94 % average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024 represents a novel species of the genus of , for which the name sp. nov. is proposed. The type strain is A7024 (=DSM 42066=KCTC 29102=NRRL B-24888).
一株新的放线菌，分离株 A7024，从土耳其西部黑海南部杜兹杰的商业榛子果园土壤样本中分离得到。进行了多相分类学研究，以确定该分离株的地位。基于 16S rRNA 基因序列构建的系统发育树表明，分离株 A7024 位于属内的成员中，与 ZFG47 的序列相似度最高（97.7%）。该生物形成广泛分支的基质和气生菌丝，产生具有光滑表面的不规则杆状孢子。菌株 A7024 的细胞壁含有 ll-二氨基庚二酸。检测到全细胞糖为葡萄糖、甘露糖和核糖。其极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、三种未鉴定的磷脂和三种未鉴定的糖脂。主要的menaquinones 为 MK-9(H) 和 MK-9(H4)。主要的细胞脂肪酸为 iso-C、anteisoC 和 anteiso-C。分离株 A7024 的基因组大小为 9.0 Mb，基因组 G+C 含量为 71.5 mol%。16S rRNA 基因相似性低（19.3±2.3%）、19.3±2.3%数字 DNA-DNA 杂交和 76.94%的平均核苷酸同一性值，以及一些不同的表型特征，使该菌株能够与密切相关的模式株区分开来。因此，我们得出结论，菌株 A7024 代表了属的一个新种，建议将其命名为 sp. nov.。模式株为 A7024（=DSM 42066=KCTC 29102=NRRL B-24888）。
