Zhang Guishan, Xu Jing, Zhang Yingnan, Yang Shubao, Jiang Huaizhi
School of Public Health, Jilin Medical University, Jilin, Jilin Province, China.
School of Basic Medical Sciences, Jilin Medical University, Jilin, Jilin Province, China.
Anim Biotechnol. 2023 Nov;34(6):1937-1942. doi: 10.1080/10495398.2022.2058519. Epub 2022 Apr 20.
MicroRNA exerts an important regulatory role in almost all the biological process, including hair follicle development in Liaoning Cashmere goat. In order to improve the Cashmere performance of goat, the regulatory role of microRNA in hair follicle cycle has drawn hotspot attention. However, the molecular mechanisms of miRNA-1-3p involved in hair follicle development are poorly understood. In this study, we found that miRNA-1-3p was less expressed in anagen stage of hair follicle cycle of Cashmere goat than that in telogen stage by using RT-qPCR and immunoblotting analysis, in contrast to the expression pattern of FGF14. The Dual-Luciferase reporter assay was employed to verify the relationship between miRNA-1-3p and FGF14. The results showed that miRNA-1-3p specifically binds to the 3'UTR of FGF14 mRNA, and FGF14 is the target gene of miR-1-3p. In conclusion, this study shows that miRNA-1-3p may regulate hair follicle development in Liaoning Cashmere goats by targeting FGF14.
微小RNA在几乎所有生物过程中都发挥着重要的调节作用，包括辽宁绒山羊的毛囊发育。为了提高山羊的羊绒性能，微小RNA在毛囊周期中的调节作用已成为热点关注对象。然而，miRNA-1-3p参与毛囊发育的分子机制尚不清楚。在本研究中，我们通过实时定量聚合酶链反应（RT-qPCR）和免疫印迹分析发现，与成纤维细胞生长因子14（FGF14）的表达模式相反，miRNA-1-3p在绒山羊毛囊周期的生长期的表达低于休止期。采用双荧光素酶报告基因检测法验证miRNA-1-3p与FGF14之间的关系。结果表明，miRNA-1-3p特异性结合FGF14 mRNA的3'非翻译区（3'UTR），且FGF14是miR-1-3p的靶基因。总之，本研究表明miRNA-1-3p可能通过靶向FGF14来调节辽宁绒山羊的毛囊发育。
